PUBLICATION
SP2 is a maternally inherited transcription factor required for embryonic development
- Authors
- Xie, J., Yin, H., Nichols, T.D., Yoder, J.A., and Horowitz, J.M.
- ID
- ZDB-PUB-091215-39
- Date
- 2010
- Source
- The Journal of biological chemistry 285(6): 4153-4164 (Journal)
- Registered Authors
- Yoder, Jeffrey A.
- Keywords
- DEVELOPMENT DIFFERENTIATION, DNA/Transcription, GENE/Promoters, GENE/Regulation, ORGANISMS/Zebra fish, TRANSCRIPTION/Development, TRANSCRIPTION/Sp1
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- COS Cells
- Chlorocebus aethiops
- Chromosome Mapping
- Embryo, Mammalian/embryology
- Embryo, Mammalian/metabolism*
- Female
- Gene Expression Regulation, Developmental
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Humans
- In Situ Hybridization
- Male
- Mice
- Microscopy, Fluorescence
- Molecular Sequence Data
- Phylogeny
- Promoter Regions, Genetic/genetics
- RNA, Messenger, Stored/genetics
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Analysis, DNA
- Sp2 Transcription Factor/classification
- Sp2 Transcription Factor/genetics*
- Sp2 Transcription Factor/metabolism
- Synteny
- Transcription, Genetic
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/growth & development
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 19959469 Full text @ J. Biol. Chem.
Citation
Xie, J., Yin, H., Nichols, T.D., Yoder, J.A., and Horowitz, J.M. (2010) SP2 is a maternally inherited transcription factor required for embryonic development. The Journal of biological chemistry. 285(6):4153-4164.
Abstract
The Sp-family of transcription factors are required for the expression of cell cycle- and developmentally-regulated genes, and the deregulated expression of a handful of family members is associated with human tumorigenesis. Sp2 is a relatively poorly characterized member of the Sp-family that, although widely expressed, exhibits little or no DNA-binding or transcriptional activity in human and mouse cell lines. To begin to address the role(s) played by Sp2 in early metazoan development we have cloned and characterized Sp2 from zebrafish (Danio rerio). We report that (1) the intron/exon organization and amino acid sequence of zebrafish Sp2 is closely conserved with its mammalian orthologues, (2) zebrafish Sp2 weakly stimulates an Sp-dependent promoter in vitro and associates with the nuclear matrix in a DNA-independent fashion, (3) zebrafish Sp2 is inherited as a maternal transcript, is transcribed in zebrafish embryos and adult tissues, and is required for completion of gastrulation, and (4) zebrafish lines carrying transgenes regulated by the Sp2 promoter recapitulate patterns of endogenous Sp2 expression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping