PUBLICATION

Arterial-venous segregation by selective cell sprouting: an alternative mode of blood vessel formation

Authors
Herbert, S.P., Huisken, J., Kim, T.N., Feldman, M.E., Houseman, B.T., Wang, R.A., Shokat, K.M., and Stainier, D.Y.
ID
ZDB-PUB-091023-30
Date
2009
Source
Science (New York, N.Y.)   326(5950): 294-298 (Journal)
Registered Authors
Stainier, Didier
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Aorta/cytology
  • Aorta/embryology
  • Arteries/cytology
  • Arteries/embryology*
  • Cell Movement
  • Endothelial Cells/cytology
  • Endothelial Cells/physiology*
  • Ephrin-B2/metabolism*
  • Morphogenesis*
  • Phosphatidylinositol 3-Kinases/metabolism
  • Receptor, EphB4/metabolism*
  • Receptors, Notch/metabolism
  • Signal Transduction
  • Stem Cells/cytology
  • Stem Cells/physiology*
  • Vascular Endothelial Growth Factor A/metabolism
  • Vascular Endothelial Growth Factor Receptor-2/metabolism
  • Vascular Endothelial Growth Factor Receptor-3/metabolism
  • Veins/cytology
  • Veins/embryology*
  • Zebrafish
  • Zebrafish Proteins/metabolism
(all 24)
PubMed
19815777 Full text @ Science
Abstract
Blood vessels form de novo (vasculogenesis) or upon sprouting of capillaries from preexisting vessels (angiogenesis). With high-resolution imaging of zebrafish vascular development, we uncovered a third mode of blood vessel formation whereby the first embryonic artery and vein, two unconnected blood vessels, arise from a common precursor vessel. The first embryonic vein formed by selective sprouting of progenitor cells from the precursor vessel, followed by vessel segregation. These processes were regulated by the ligand EphrinB2 and its receptor EphB4, which are expressed in arterial-fated and venous-fated progenitors, respectively, and interact to orient the direction of progenitor migration. Thus, directional control of progenitor migration drives arterial-venous segregation and generation of separate parallel vessels from a single precursor vessel, a process essential for vascular development.
Genes / Markers
Marker Marker Type Name
dll4GENEdelta-like 4 (Drosophila)
efnb2aGENEephrin-B2a
ephb4aGENEeph receptor B4a
flt4GENEfms related receptor tyrosine kinase 4
hey2GENEhes-related family bHLH transcription factor with YRPW motif 2
kdrlGENEkinase insert domain receptor like
notch1aGENEnotch receptor 1a
plcg1GENEphospholipase C, gamma 1
tnnt2aGENEtroponin T type 2a (cardiac)
vegfaaGENEvascular endothelial growth factor Aa
1 - 10 of 11
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Figures
No images available
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
DsRedsd2Tgstandard conditionsPrim-5IFL
EGFPs843Tgstandard conditionsPrim-5IFL
EGFPs843Tgstandard conditions26+ somitesIFL
1 - 3 of 3
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Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-efnb2astandard conditions26+ somites
WT + MO1-efnb2astandard conditions26+ somites
WT + MO1-ephb4astandard conditions26+ somites
WT + MO1-ephb4astandard conditions26+ somites
WT + MO1-flt4standard conditionsLong-pec
WT + MO1-flt4standard conditionsLong-pec
WT + MO1-flt4 + MO3-plcg1standard conditionsLong-pec
WT + MO1-flt4 + MO3-plcg1standard conditionsLong-pec
WT + MO1-hey2standard conditionsPrim-5 to High-pec
WT + MO1-hey2standard conditionsPrim-5 to High-pec
1 - 10 of 23
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
s843TgTransgenic Insertion
    sd2TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      efnb2aMO1-efnb2aMRPHLNO
      ephb4aMO1-ephb4aMRPHLNO
      flt4MO1-flt4MRPHLNO
      hey2MO1-hey2MRPHLNO
      plcg1MO3-plcg1MRPHLNO
      tnnt2aMO1-tnnt2aMRPHLNO
      vegfaaMO3-vegfaaMRPHLNO
      vegfcMO1-vegfcMRPHLNO
      1 - 8 of 8
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      Fish
      Fish
      s843Tg
      s843Tg; sd2Tg
      sd2Tg
      WT + MO1-efnb2a
      WT + MO1-ephb4a
      WT + MO1-flt4
      WT + MO1-flt4 + MO3-plcg1
      WT + MO1-hey2
      WT + MO1-tnnt2a
      WT + MO3-plcg1
      1 - 10 of 11
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      Antibodies
      Orthology
      Engineered Foreign Genes
      Marker Marker Type Name
      DsRedEFGDsRed
      EGFPEFGEGFP
      1 - 2 of 2
      Show
      Mapping
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      Citation
      Herbert, S.P., Huisken, J., Kim, T.N., Feldman, M.E., Houseman, B.T., Wang, R.A., Shokat, K.M., and Stainier, D.Y. (2009) Arterial-venous segregation by selective cell sprouting: an alternative mode of blood vessel formation. Science (New York, N.Y.). 326(5950):294-298.