PUBLICATION
Characterization, expression, and response to stress of p8 gene in amphioxus
- Authors
- Liu, Z., Sun, Y., Liu, N., Fan, N., and Zhang, S.
- ID
- ZDB-PUB-090706-3
- Date
- 2009
- Source
- Fish & shellfish immunology 27(3): 407-413 (Journal)
- Registered Authors
- Liu, Zhenhui
- Keywords
- Amphioxus, p8, Expression, LPS, Starvation
- MeSH Terms
-
- Adjuvants, Immunologic/pharmacology
- Amino Acid Sequence
- Animals
- Base Sequence
- Chordata, Nonvertebrate/classification
- Chordata, Nonvertebrate/genetics*
- Chordata, Nonvertebrate/metabolism*
- DNA-Binding Proteins/chemistry
- DNA-Binding Proteins/genetics*
- DNA-Binding Proteins/metabolism*
- Gene Expression Regulation/drug effects
- Humans
- Lipopolysaccharides/pharmacology
- Male
- Mice
- Molecular Sequence Data
- Phylogeny
- RNA, Messenger/metabolism
- Rats
- Sequence Homology, Amino Acid
- Stress, Physiological/physiology*
- PubMed
- 19560542 Full text @ Fish Shellfish Immunol.
Citation
Liu, Z., Sun, Y., Liu, N., Fan, N., and Zhang, S. (2009) Characterization, expression, and response to stress of p8 gene in amphioxus. Fish & shellfish immunology. 27(3):407-413.
Abstract
The amphioxus Branchiostoma belcheri tsingtaunese homolog of p8, Bbp8, was identified from the gut cDNA library. The full-length Bbp8 cDNA consists of 1032bp, which is clearly longer than those of p8 in human, mouse, rat, frog, zebrafish and fruit fly. The genomic DNA sequences of amphioxus p8 contain three exons and two introns, which is similar to the exon/intron organization of p8 homologues in vertebrates such as human, mouse and zebrafish, while it is sharply different to the organization of p8 gene in fruit fly, which has only one exon. Sequence alignment and phylogenetic analysis showed that the basic helix-loop-helix (bHLH) region of p8 is well conserved during the long process of evolution, and Bbp8 is more close to its homologous proteins in the invertebrates than to those in the vertebrates. RT-PCR and In situ hybridization histochemistry demonstrated the expression of Bbp8 in all the tissues assayed, with relatively higher expression in the gut, gill and ovaries. Quantitative real-time PCR assay revealed quick up-regulation of Bbp8 transcripts on lipopolysaccharide (LPS) challenge and starvation, implying a stress-related function for Bbp8.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping