PUBLICATION
Identification of vasculature-specific genes by microarray analysis of etsrp/etv2 overexpressing zebrafish embryos
- Authors
- Wong, K.S., Proulx, K., Rost, M.S., and Sumanas, S.
- ID
- ZDB-PUB-090616-41
- Date
- 2009
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 238(7): 1836-1850 (Journal)
- Registered Authors
- Sumanas, Saulius
- Keywords
- zebrafish, vasculogenesis, angiogenesis, vascular endothelial, microarray, etsrp, etv2, ER71, myeloid
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Blood Vessels/embryology
- Blood Vessels/metabolism*
- Embryo, Nonmammalian
- Gene Expression Profiling
- Gene Expression Regulation, Developmental
- Oligonucleotide Array Sequence Analysis
- Organ Specificity/genetics
- Up-Regulation
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- PubMed
- 19504456 Full text @ Dev. Dyn.
Citation
Wong, K.S., Proulx, K., Rost, M.S., and Sumanas, S. (2009) Identification of vasculature-specific genes by microarray analysis of etsrp/etv2 overexpressing zebrafish embryos. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(7):1836-1850.
Abstract
Signaling pathways controlling vasculogenesis, angiogenesis, and myelopoiesis are still poorly understood, in part because not all genes important for vasculature or myeloid cell formation have been characterized. To identify novel potential regulators of vasculature and myeloid cell formation we performed microarray analysis of zebrafish embryos that overexpress Ets1-related protein (Etsrp/Etv2/ER71), sufficient to induce vasculogenesis and myelopoiesis (Sumanas and Lin [2006] Development 121:3141-3150; Lee [2008] Cell Stem Cell 2:497-507; Sumanas et al. [2008] Blood 111:4500-4510). We performed sequence homology and expression analysis for up-regulated genes that were novel or previously unassociated with the zebrafish vasculature formation. Angiotensin II type 2 receptor (agtr2), src homology 2 domain containing E (she), mannose receptor C1 (mrc1), endothelial cell-specific adhesion molecule (esam), yes-related kinase (yrk/fyn), zinc finger protein, multitype 2b (zfpm2b/fog2b), and stabilin 2 (stab2) were specifically expressed in vascular endothelial cells during early development while keratin18 expression was localized to the myeloid cells. Identification of vasculature and myeloid-specific genes will be important for dissecting molecular mechanisms of vasculogenesis/angiogenesis and myelopoiesis.
Genes / Markers
Probes
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping