PUBLICATION
Channel catfish (Ictalurus punctatus Rafinesque, 1818) CD156a (ADAM metallopeptidase domain 8): cDNA clone, characterization and expression in tissues
- Authors
- Yeh, H.Y., and Klesius, P.H.
- ID
- ZDB-PUB-090616-34
- Date
- 2009
- Source
- Veterinary Immunology and Immunopathology 132(2-4): 307-313 (Journal)
- Registered Authors
- Keywords
- CD156a, A disintegrin and metalloprotease domain 8, ADAM metallopeptidase domain 8 (ADAM-8), Channel catfish, Ictalurus punctatus
- MeSH Terms
-
- ADAM Proteins/chemistry
- ADAM Proteins/genetics*
- ADAM Proteins/immunology*
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- Conserved Sequence
- DNA Primers/genetics
- DNA, Complementary/genetics
- Gene Expression Profiling
- Ictaluridae/genetics*
- Ictaluridae/immunology*
- Molecular Sequence Data
- Molecular Weight
- Protein Structure, Tertiary
- Sequence Homology, Amino Acid
- Species Specificity
- Tissue Distribution
- PubMed
- 19501921 Full text @ Vet. Immunol. Immunopathol.
Citation
Yeh, H.Y., and Klesius, P.H. (2009) Channel catfish (Ictalurus punctatus Rafinesque, 1818) CD156a (ADAM metallopeptidase domain 8): cDNA clone, characterization and expression in tissues. Veterinary Immunology and Immunopathology. 132(2-4):307-313.
Abstract
CD156a, also known as a disintegrin and metalloprotease domain 8 (ADAM-8), is a type 1 transmembrane glycoprotein of the ADAM family. This protein plays important roles in immune and other physiological functions. In this communication, the channel catfish CD156a cDNA was characterized and its expression in various tissues was determined. The full-length of channel catfish CD156a cDNA had 3035 nucleotides, including an open reading frame which appears to encode an 850 amino acid peptide with a calculated molecular mass of 94.6kDa. The peptide had three potential N-glycosylation sites. By comparison with other species, the degree of homology of the CD156a amino acid sequences ranged from 31.6% (vs. chicken CD156a) to 59.5% (vs. zebrafish CD156a). The channel catfish CD156a peptide could be structurally divided into nine domains. Several canonical features for CD156a functions were conserved in channel catfish. The CD156a transcript was detected by two-step RT-PCR in anterior kidney and gill, suggesting that CD156a may be involved in the innate immune response in channel catfish. Reagents for further elucidating the immune functions of channel catfish CD156a are under development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping