PUBLICATION
miR-8 microRNAs regulate the response to osmotic stress in zebrafish embryos
- Authors
- Flynt, A.S., Thatcher, E.J., Burkewitz, K., Li, N., Liu, Y., and Patton, J.G.
- ID
- ZDB-PUB-090407-4
- Date
- 2009
- Source
- The Journal of cell biology 185(1): 115-127 (Journal)
- Registered Authors
- Flynt, Alex, Li, Nan, Patton, James G., Thatcher, Elizabeth
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Biological Transport
- Embryo, Nonmammalian/physiology*
- Epistasis, Genetic
- Homeostasis
- MicroRNAs/physiology*
- Osmotic Pressure*
- Phosphoproteins/genetics
- Phosphoproteins/metabolism
- Phylogeny
- Sequence Alignment
- Sodium/metabolism
- Sodium-Hydrogen Exchangers/genetics
- Sodium-Hydrogen Exchangers/metabolism
- Stress, Physiological*
- Zebrafish/genetics
- Zebrafish/physiology*
- PubMed
- 19332888 Full text @ J. Cell Biol.
Citation
Flynt, A.S., Thatcher, E.J., Burkewitz, K., Li, N., Liu, Y., and Patton, J.G. (2009) miR-8 microRNAs regulate the response to osmotic stress in zebrafish embryos. The Journal of cell biology. 185(1):115-127.
Abstract
MicroRNAs (miRNAs) are highly conserved small RNAs that act as translational regulators of gene expression, exerting their influence by selectively targeting mRNAs bearing complementary sequence elements. These RNAs function in diverse aspects of animal development and physiology. Because of an ability to act as rapid responders at the level of translation, miRNAs may also influence stress response. In this study, we show that the miR-8 family of miRNAs regulates osmoregulation in zebrafish embryos. Ionocytes, which are a specialized cell type scattered throughout the epidermis, are responsible for pH and ion homeostasis during early development before gill formation. The highly conserved miR-8 family is expressed in ionocytes and enables precise control of ion transport by modulating the expression of Nherf1, which is a regulator of apical trafficking of transmembrane ion transporters. Ultimately, disruption of miR-8 family member function leads to an inability to respond to osmotic stress and blocks the ability to properly traffic and/or cluster transmembrane glycoproteins at the apical surface of ionocytes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping