PUBLICATION

Estrogen receptor subtype beta2 is involved in neuromast development in zebrafish (Danio rerio) larvae

Authors
Froehlicher, M., Liedtke, A., Groh, K., Lopez-Schier, H., Neuhauss, S.C., Segner, H., and Eggen, R.I.
ID
ZDB-PUB-090319-13
Date
2009
Source
Developmental Biology   330(1): 32-43 (Journal)
Registered Authors
Lopez-Schier, Hernan, Neuhauss, Stephan
Keywords
Estrogen Receptor, Neuromast, Zebrafish, Notch Signaling
Datasets
GEO:GSE13158, GEO:GSE13157, GEO:GSE13771
MeSH Terms
  • Animals
  • Embryo, Nonmammalian/metabolism
  • Female
  • Larva/metabolism
  • Neurons, Afferent/metabolism*
  • RNA, Messenger/metabolism
  • Receptors, Estrogen/genetics
  • Receptors, Estrogen/metabolism*
  • Receptors, Notch/metabolism
  • Zebrafish/embryology
  • Zebrafish/growth & development*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 13)
PubMed
19289112 Full text @ Dev. Biol.
Abstract
Estrogens are known to play a role in both reproductive and non-reproductive functions in mammals. Estrogens and their receptors are involved in the development of the central nervous system (brain development, neuronal survival and differentiation) as well as in the development of the peripheral nervous system (sensory-motor behaviors). In order to decipher possible functions of estrogens in early development of the zebrafish sensory system, we investigated the role of estrogen receptor beta(2) (ERbeta(2)) by using a morpholino (MO) approach blocking erbeta(2) RNA translation. We further investigated the development of lateral line organs by cell-specific labeling, which revealed a disrupted development of neuromasts in morphants. The supporting cells developed and migrated normally. Sensory hair cells, however, were absent in morphants' neuromasts. Microarray analysis and subsequent in situ hybridizations indicated an aberrant activation of the Notch signaling pathway in ERbeta(2) morphants. We conclude that signaling via ERbeta(2) is essential for hair cell development and may involve an interaction with the Notch signaling pathway during cell fate decision in the neuromast maturation process.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-esr2astandard conditionsPrim-5 to Day 4
WT + MO1-esr2astandard conditionsProtruding-mouth
WT + MO1-esr2astandard conditionsProtruding-mouth
WT + MO1-esr2astandard conditionsProtruding-mouth to Day 5
WT + MO1-esr2astandard conditionsProtruding-mouth to Day 5
WT + MO1-esr2astandard conditionsDay 4
WT + MO1-esr2astandard conditionsLong-pec to Day 4
WT + MO1-esr2astandard conditionsProtruding-mouth
WT + MO1-esr2astandard conditionsProtruding-mouth
WT + MO1-esr2astandard conditionsProtruding-mouth
1 - 10 of 13
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sqet4EtTransgenic Insertion
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    esr2aMO1-esr2aMRPHLNO
    tp53MO4-tp53MRPHLNO
    1 - 2 of 2
    Show
    Fish
    Fish
    sqet4Et
    sqet4Et + MO1-esr2a
    WT
    WT + MO1-esr2a
    WT + MO1-esr2a + MO4-tp53
    1 - 5 of 5
    Show
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
    1 - 1 of 1
    Show
    Mapping
    No data available
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    Citation
    Froehlicher, M., Liedtke, A., Groh, K., Lopez-Schier, H., Neuhauss, S.C., Segner, H., and Eggen, R.I. (2009) Estrogen receptor subtype beta2 is involved in neuromast development in zebrafish (Danio rerio) larvae. Developmental Biology. 330(1):32-43.