PUBLICATION
Zebrafish granulocyte colony-stimulating factor receptor signaling promotes myelopoiesis and myeloid cell migration
- Authors
- Liongue, C., Hall, C.J., O'Connell, B.A., Crosier, P., and Ward, A.C.
- ID
- ZDB-PUB-090116-21
- Date
- 2009
- Source
- Blood 113(11): 2535-2546 (Journal)
- Registered Authors
- Crosier, Phil, Hall, Chris, Liongue, Clifford, Ward, Alister C.
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Animals, Genetically Modified
- Cell Movement/genetics*
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental
- Hematopoiesis/genetics
- Molecular Sequence Data
- Myeloid Cells/metabolism
- Myeloid Cells/physiology*
- Myeloid Progenitor Cells/metabolism
- Myeloid Progenitor Cells/physiology
- Myelopoiesis/genetics*
- Phylogeny
- Receptors, Granulocyte Colony-Stimulating Factor/genetics*
- Receptors, Granulocyte Colony-Stimulating Factor/metabolism
- Receptors, Granulocyte Colony-Stimulating Factor/physiology
- Sequence Homology, Amino Acid
- Signal Transduction/genetics
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/physiology
- PubMed
- 19139076 Full text @ Blood
Citation
Liongue, C., Hall, C.J., O'Connell, B.A., Crosier, P., and Ward, A.C. (2009) Zebrafish granulocyte colony-stimulating factor receptor signaling promotes myelopoiesis and myeloid cell migration. Blood. 113(11):2535-2546.
Abstract
Granulocyte colony-stimulating factor receptor (GCSFR) signaling participates in the production of neutrophilic granulocytes during normal hematopoietic development, with a particularly important role during emergency hematopoiesis. This study describes the characterization of the zebrafish gcsf and gcsfr genes, which showed broad conservation and similar regulation to their mammalian counterparts. Morpholino-mediated knockdown of gcsfr and overexpression of gcsf revealed the presence of an anterior population of myeloid cells during primitive hematopoiesis that were dependent on GCSF/GCSFR for their development and migration. This contrasted with a posterior domain that was largely independent of this pathway. Definitive myelopoiesis was also partially dependent on a functional GCSF/GCSFR pathway. Injection of bacterial lipopolysaccharide elicited significant induction of gcsf expression and emergency production of myeloid cells, which was abrogated by gcsfr knockdown. Collectively, these data demonstrate GCSF/GCSFR to be a conserved signaling system for facilitating the production of multiple myeloid cell lineages in both homeostatic and emergency conditions, as well as for early myeloid cell migration, establishing a useful experimental platform for further dissection of this pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping