PUBLICATION
Evidence for two distinct KiSS genes in non-placental vertebrates that encode kisspeptins with different gonadotropin-releasing activities in fish and mammals
- Authors
- Felip, A., Zanuy, S., Pineda, R., Pinilla, L., Carrillo, M., Tena-Sempere, M., and Gómez, A.
- ID
- ZDB-PUB-081218-32
- Date
- 2009
- Source
- Molecular and Cellular Endocrinology 312(1-2): 61-71 (Journal)
- Registered Authors
- Keywords
- KiSS-1, KiSS-2, vertebrate evolution, LH, FSH, Dicentrarchus labrax, Rattus norvegicus
- MeSH Terms
-
- Aging
- Amino Acid Sequence
- Analysis of Variance
- Animals
- Base Sequence
- Bass/genetics
- Bass/metabolism
- Cloning, Molecular
- Female
- Fish Proteins/chemistry
- Fish Proteins/genetics*
- Fish Proteins/metabolism
- Gonadotropins, Pituitary/blood
- Kisspeptins
- Male
- Molecular Sequence Data
- Organ Specificity
- Oryzias/genetics
- Oryzias/metabolism
- Phylogeny
- Proteins/chemistry
- Proteins/genetics*
- Proteins/metabolism
- Rats
- Rats, Wistar
- Reproduction/genetics*
- Seasons
- Sequence Alignment
- Sequence Analysis, DNA
- Synteny
- Time Factors
- Vertebrates/genetics*
- Vertebrates/metabolism
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 19084576 Full text @ Mol. Cell. Endocrinol.
Citation
Felip, A., Zanuy, S., Pineda, R., Pinilla, L., Carrillo, M., Tena-Sempere, M., and Gómez, A. (2009) Evidence for two distinct KiSS genes in non-placental vertebrates that encode kisspeptins with different gonadotropin-releasing activities in fish and mammals. Molecular and Cellular Endocrinology. 312(1-2):61-71.
Abstract
Kisspeptins, the products of KiSS-1 gene, have recently emerged as fundamental regulators of reproductive function in different mammalian and, presumably, non-mammalian species. To date, a single form of KiSS-1 has been described in mammals, and recently, in several fish species and Xenopus. We report herein the cloning and characterization of two distinct KiSS-like genes, namely, KiSS-1 and KiSS-2, in the teleost sea bass. While KiSS-1 encodes a peptide identical to rodent kisspeptin-10, the predicted KiSS-2 decapeptide diverges at 4 amino acids (FNFNPFGLRF). Genome database searches showed that both genes are present in non-placental vertebrate genomes. Indeed, phylogenetic and genome mapping analyses suggest that KiSS-1 and KiSS-2 are paralogous genes that originated by duplication of an ancestral gene, although KiSS-2 is lost in placental mammals. KiSS-1 and KiSS-2 mRNAs are present in brain and gonads of sea bass, medaka and zebrafish. Comparative functional studies demonstrated that KiSS-2 decapeptide was significantly more potent than KiSS-1 peptide in inducing LH and FSH secretion in sea bass. In contrast, KiSS-2 decapeptide only weakly elicited LH secretion in rats, whereas KiSS-1 peptide was maximally effective. Our data are the first to provide conclusive evidence for the existence of a second KiSS gene, KiSS-2, in non-placental vertebrates, whose product is likely to play a dominant stimulatory role in the regulation of the gonadotropic axis at least in teleosts.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping