PUBLICATION
A single method for cryofixation and correlative light, electron microscopy and tomography of zebrafish embryos
- Authors
- Nixon, S.J., Webb, R.I., Floetenmeyer, M., Schieber, N., Lo, H.P., and Parton, R.G.
- ID
- ZDB-PUB-081217-6
- Date
- 2009
- Source
- Traffic (Copenhagen, Denmark) 10(2): 131-136 (Journal)
- Registered Authors
- Lo, Harriet, Parton, Robert G.
- Keywords
- none
- MeSH Terms
-
- Animals
- Cryopreservation/methods*
- Microscopy/methods*
- Tomography/methods*
- Zebrafish/embryology*
- PubMed
- 19054388 Full text @ Traffic
Citation
Nixon, S.J., Webb, R.I., Floetenmeyer, M., Schieber, N., Lo, H.P., and Parton, R.G. (2009) A single method for cryofixation and correlative light, electron microscopy and tomography of zebrafish embryos. Traffic (Copenhagen, Denmark). 10(2):131-136.
Abstract
The zebrafish is a powerful vertebrate system for cell and developmental studies. Here we have optimized methods for fast freezing and processing of zebrafish embryos for electron microscopy. We show that in the absence of primary chemical fixation excellent ultrastructure, preservation of GFP fluorescence, immunogold labeling, and electron tomography, can be obtained using a single technique involving high pressure freezing and embedding in lowicryl resins at low temperature. As well as being an important new tool for zebrafish research, the maintenance of GFP fluorescence after fast freezing, freeze substitution, and resin embedding, will be of general use for correlative light and electron microscopy of biological samples.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping