PUBLICATION
Zebrafish Dorsal Root Ganglia Neural Precursor Cells Adopt a Glial Fate in the Absence of Neurogenin1
- Authors
- McGraw, H.F., Nechiporuk, A., and Raible, D.W.
- ID
- ZDB-PUB-081121-26
- Date
- 2008
- Source
- The Journal of neuroscience : the official journal of the Society for Neuroscience 28(47): 12558-12569 (Journal)
- Registered Authors
- McGraw, Hillary, Nechiporuk, Alex, Raible, David
- Keywords
- dorsal root ganglia, neural crest, fate restriction, neurogenin 1, sensory neuron, glia
- MeSH Terms
-
- Analysis of Variance
- Animals
- Animals, Genetically Modified
- Basic Helix-Loop-Helix Transcription Factors/deficiency*
- Basic Helix-Loop-Helix Transcription Factors/genetics
- Body Patterning/genetics
- Cell Cycle/drug effects
- Cell Differentiation/drug effects
- Cell Proliferation
- Chromosomes, Artificial, Bacterial/genetics
- Embryo, Nonmammalian
- Ganglia, Spinal/cytology*
- Ganglia, Spinal/embryology
- Gene Expression Regulation, Developmental/genetics
- Green Fluorescent Proteins/biosynthesis
- Green Fluorescent Proteins/genetics
- Morpholines/pharmacology
- Mutation/genetics
- Nerve Tissue Proteins/deficiency*
- Nerve Tissue Proteins/genetics
- Neuroglia/drug effects
- Neuroglia/physiology*
- Neurons/cytology
- Neurons/drug effects
- Neurons/physiology*
- SOXE Transcription Factors/genetics
- SOXE Transcription Factors/metabolism
- Stem Cells/drug effects
- Stem Cells/physiology*
- Time Factors
- Veratrum Alkaloids/pharmacology
- Zebrafish
- Zebrafish Proteins/deficiency*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 19020048 Full text @ J. Neurosci.
Citation
McGraw, H.F., Nechiporuk, A., and Raible, D.W. (2008) Zebrafish Dorsal Root Ganglia Neural Precursor Cells Adopt a Glial Fate in the Absence of Neurogenin1. The Journal of neuroscience : the official journal of the Society for Neuroscience. 28(47):12558-12569.
Abstract
The proneural transcription factor neurogenin 1 (neurog1) has been shown to be a key regulator of dorsal root ganglion (DRG) neuron development. Here we use a novel transgenic zebrafish line to demonstrate that the neural crest population that gives rise to DRG neurons becomes fate restricted to a neuronal/glial precursor before the onset of neurog1 function. We generated a stable transgenic zebrafish line that carries a modified bacterial artificial chromosome that expresses green fluorescent protein (GFP) under the control of the neurog1 promoter [Tg(neurog1:EGFP)]. In contrast to previously described neurog1 transgenic lines, Tg(neurog1:EGFP) expresses GFP in DRG neuronal precursors cells as they migrate ventrally and after their initial differentiation as neurons. Using this line, we are able to track the fate of DRG neuronal precursor cells during their specification. When Neurog1 function is blocked, either by neurog1 morpholino antisense oligonucleotide injection or in neurog1 mutants, GFP expression initiates in neural crest cells, although they fail to form DRG neurons. Rather, these cells take on a glial-like morphology, retain proliferative capacity, and express glial markers and become associated with the ventral motor root. These results suggest that, within the zebrafish neural crest, there is a fate-restricted lineage that is limited to form either sensory neurons or glia in the developing DRG. Neurog1 acts as the key factor in this lineage to direct the formation of sensory neurons.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping