PUBLICATION
Cell surface expression of channel catfish leukocyte immune-type receptors (IpLITRs) and recruitment of both Src homology 2 domain-containing protein tyrosine phosphatase (SHP)-1 and SHP-2
- Authors
- Montgomery, B.C., Mewes, J., Davidson, C., Burshtyn, D.N., and Stafford, J.L.
- ID
- ZDB-PUB-081121-14
- Date
- 2009
- Source
- Developmental and comparative immunology 33(4): 570-582 (Journal)
- Registered Authors
- Keywords
- Innate immunity, Immunoglobulin superfamily, Receptors, Signaling, Natural killer cells, Tyrosine phosphorylation, Cellular inhibition, Phosphatases
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line, Tumor
- Cell Membrane/immunology*
- Cell Membrane/metabolism
- HeLa Cells
- Humans
- Ictaluridae/genetics
- Ictaluridae/immunology*
- Leukocytes/immunology
- Leukocytes/metabolism
- Mice
- Molecular Sequence Data
- Protein Tyrosine Phosphatase, Non-Receptor Type 11/genetics
- Protein Tyrosine Phosphatase, Non-Receptor Type 11/metabolism*
- Protein Tyrosine Phosphatase, Non-Receptor Type 6/genetics
- Protein Tyrosine Phosphatase, Non-Receptor Type 6/metabolism*
- Receptors, Immunologic/genetics
- Receptors, Immunologic/immunology
- Receptors, Immunologic/metabolism*
- Sequence Alignment
- Transfection
- Zebrafish/embryology
- Zebrafish/genetics
- src Homology Domains/immunology
- PubMed
- 19013191 Full text @ Dev. Comp. Immunol.
Citation
Montgomery, B.C., Mewes, J., Davidson, C., Burshtyn, D.N., and Stafford, J.L. (2009) Cell surface expression of channel catfish leukocyte immune-type receptors (IpLITRs) and recruitment of both Src homology 2 domain-containing protein tyrosine phosphatase (SHP)-1 and SHP-2. Developmental and comparative immunology. 33(4):570-582.
Abstract
Channel catfish leukocyte immune-type receptors (IpLITRs) are immunoglobulin superfamily (IgSF) members believed to play a role in the control and coordination of cellular immune responses in teleost. Putative stimulatory and inhibitory IpLITRs are co-expressed by different types of catfish immune cells (e.g. NK cells, T cells, B cells, and macrophages) but their signaling potential has not been determined. Following cationic polymer-mediated transfections into human cell lines we examined the surface expression, tyrosine phosphorylation, and phosphatase recruitment potential of two types of putative inhibitory IpLITRs using 'chimeric' expression constructs and an epitope-tagged 'native' IpLITR. We also cloned and expressed the teleost Src homology 2 domain-containing protein tyrosine phosphatases (SHP)-1 and SHP-2 and examined their expression in adult tissues and developing zebrafish embryos. Co-immunoprecipitation experiments support the inhibitory signaling potential of distinct IpLITR-types that bound both SHP-1 and SHP-2 following the phosphorylation of tyrosine residues within their cytoplasmic tail (CYT) regions. Phosphatase recruitment by IpLITRs represents an important first step in understanding their influence on immune cell effector functions and suggests that certain inhibitory signaling pathways are conserved among vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping