PUBLICATION
Alpha2 macroglobulin-like is essential for liver development in zebrafish
- Authors
- Hong, S.K., and Dawid, I.B.
- ID
- ZDB-PUB-081121-13
- Date
- 2008
- Source
- PLoS One 3(11): e3736 (Journal)
- Registered Authors
- Dawid, Igor B., Hong, Sung-Kook
- Keywords
- Embryos, Zebrafish, Pancreas, Untranslated regions, Gastrointestinal tract, Hematoxylin staining, In situ hybridization, Xenopus
- MeSH Terms
-
- Animals
- Cell Proliferation
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Gastrointestinal Tract/embryology
- Gastrointestinal Tract/metabolism
- Gene Expression Regulation, Developmental
- Hepatocytes/cytology
- In Situ Hybridization
- In Situ Nick-End Labeling
- Liver/cytology
- Liver/embryology*
- Liver/metabolism*
- Oligonucleotides, Antisense/pharmacology
- Organ Specificity
- Organogenesis
- Pancreas/embryology
- Pancreas/metabolism
- Phenotype
- Phylogeny
- Zebrafish/embryology*
- Zebrafish/genetics*
- alpha-Macroglobulins/genetics*
- alpha-Macroglobulins/metabolism
- PubMed
- 19011686 Full text @ PLoS One
Citation
Hong, S.K., and Dawid, I.B. (2008) Alpha2 macroglobulin-like is essential for liver development in zebrafish. PLoS One. 3(11):e3736.
Abstract
BACKGROUND: Alpha 2 Macroglobulin family members have been studied extensively with respect to their roles in physiology and human disease including innate immunity and Alzheimer's disease, but little is known about a possible role in liver development loss-of-function in model systems.
PRINCIPAL FINDINGS: We report the isolation of the zebrafish alpha2 macroglobulin-like (A2ML) gene and its specific expression in the liver during differentiation. Morpholino-based knock-down of A2ML did not block the initial formation of the liver primordium, but inhibited liver growth and differentiation.
SIGNIFICANCE: This report on A2ML function in zebrafish development provides the first evidence for a specific role of an A2M family gene in liver formation during early embryogenesis in a vertebrate.
PRINCIPAL FINDINGS: We report the isolation of the zebrafish alpha2 macroglobulin-like (A2ML) gene and its specific expression in the liver during differentiation. Morpholino-based knock-down of A2ML did not block the initial formation of the liver primordium, but inhibited liver growth and differentiation.
SIGNIFICANCE: This report on A2ML function in zebrafish development provides the first evidence for a specific role of an A2M family gene in liver formation during early embryogenesis in a vertebrate.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping