PUBLICATION

Protective effects of vitamin E against 3,3′,4,4′,5-pentachlorobiphenyl (PCB126) induced toxicity in zebrafish embryos

Authors
Na, Y.R., Seok, S.H., Baek, M.W., Lee, H.Y., Kim, D.J., Park, S.H., Lee, H.K., and Park, J.H.
ID
ZDB-PUB-081105-3
Date
2009
Source
Ecotoxicology and environmental safety   72(3): 714-719 (Journal)
Registered Authors
Keywords
3, 3′, 4, 4′, 5-Pentachlorobiphenyl (PCB126), Vitamin E, Zebrafish (Danio rerio) embryo, CYP1A, HSC70, SOD2
MeSH Terms
  • Animals
  • Antioxidants/pharmacology*
  • Drug Antagonism
  • Edema/chemically induced
  • Edema/pathology
  • Embryo, Nonmammalian/drug effects*
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/physiopathology
  • Embryonic Development/drug effects
  • Estrogen Antagonists/toxicity*
  • Gene Expression/drug effects
  • Oxidative Stress/drug effects
  • Pericardium/drug effects
  • Pericardium/pathology
  • Polychlorinated Biphenyls/toxicity*
  • RNA, Messenger/metabolism
  • Vitamin E/pharmacology*
  • Water Pollutants, Chemical/toxicity*
  • Yolk Sac/drug effects
  • Yolk Sac/pathology
  • Zebrafish*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
18973944 Full text @ Ecotoxicol. Environ. Saf.
Abstract
3,3',4,4',5-Pentachlorinated biphenyls 126 (PCB126) is a global environmental contaminant that can induce cellular oxidative stress. We investigated whether vitamin E can protect against toxicity from PCB126 during zebrafish (Danio rerio) development. Zebrafish embryos were exposed to 100nM PCB126 and compared with a second group that was co-exposed with 100muM vitamin E until 5 days post fertilization. PCB126 induced pericardial sac edema, yolk sac edema, and growth retardation in zebrafish embyos. In contrast, vitamin E co-exposure group did not show any gross changes. Real-time PCR results showed that vitamin E co-exposure group were restored to control group for the expression levels of heat shock protein 70 Cognate, aryl hydrocarbon receptor type-2, cytochrome P450 1A, and superoxide dismutase-1. These data give insights into the use of vitamin E to reduce PCB126-mediated toxicity and into the use of zebrafish embryos for exploring mechanisms underlying the oxidative potential of AHR agonists.
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