PUBLICATION
Formation of the asymmetric pineal complex in zebrafish requires two independently acting transcription factors
- Authors
- Snelson, C.D., Burkart, J.T., and Gamse, J.T.
- ID
- ZDB-PUB-080722-13
- Date
- 2008
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 237(12): 3538-3544 (Journal)
- Registered Authors
- Gamse, Josh, Snelson, Corey
- Keywords
- homeodomain, t-box, epiphysis, epithalamus, dorsal diencephalon
- MeSH Terms
-
- Animals
- Body Patterning*
- Cell Division
- Gene Expression Regulation, Developmental
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism*
- Mutation/genetics
- Phenotype
- Pineal Gland/cytology
- Pineal Gland/embryology*
- Pineal Gland/metabolism*
- T-Box Domain Proteins/genetics
- T-Box Domain Proteins/metabolism*
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 18629869 Full text @ Dev. Dyn.
Citation
Snelson, C.D., Burkart, J.T., and Gamse, J.T. (2008) Formation of the asymmetric pineal complex in zebrafish requires two independently acting transcription factors. Developmental Dynamics : an official publication of the American Association of Anatomists. 237(12):3538-3544.
Abstract
The pineal complex of zebrafish consists of a pineal organ and a left-sided parapineal organ. Mutation of the floating head (flh) gene, which encodes a homeodomain protein, causes premature termination of pineal cell division without affecting specification or asymmetric placement of the parapineal. The from beyond (fby) mutation, a premature stop codon in the T-domain-containing protein Tbx2b, disrupts formation of the parapineal while leaving the pineal largely intact. However, flh is reported as being required for tbx2b transcription. To resolve the paradox that flhand tbx2b mutants have opposite phenotypes but have been placed in the same genetic pathway, we have examined transcriptional cross-regulation in single flh or fby mutants and genetic epistasis in double mutants. Careful analysis shows that flh is not required for tbx2b transcription and double mutants exhibit an additive phenotype. We conclude that Flh and Tbx2b regulate separate programs of pineal and parapineal development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping