PUBLICATION
LPA1 is essential for lymphatic vessel development in zebrafish
- Authors
- Lee, S.J., Chan, T.H., Chen, T.C., Liao, B.K., Hwang, P.P., and Lee, H.
- ID
- ZDB-PUB-080714-11
- Date
- 2008
- Source
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22(10): 3706-3715 (Journal)
- Registered Authors
- Hwang, Pung Pung
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- Embryonic Development/genetics
- Molecular Sequence Data
- Oligonucleotides, Antisense/genetics
- Phylogeny
- RNA Splicing
- Receptors, Lysophosphatidic Acid/classification
- Receptors, Lysophosphatidic Acid/genetics
- Receptors, Lysophosphatidic Acid/physiology*
- Sequence Analysis, Protein
- Thoracic Duct/embryology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/classification
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- PubMed
- 18606866 Full text @ FASEB J.
Citation
Lee, S.J., Chan, T.H., Chen, T.C., Liao, B.K., Hwang, P.P., and Lee, H. (2008) LPA1 is essential for lymphatic vessel development in zebrafish. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 22(10):3706-3715.
Abstract
Lysophosphatidic acid (LPA) has long been implicated in regulating vascular development via endothelial cell-expressed G protein-coupled receptors. However, because of a lack of notable vascular defects reported in LPA receptor knockout mouse studies, the regulation of vasculature by LPA receptors in vivo is still uncertain. Using zebrafish as a model, we studied the gene expression patterns and functions of an LPA receptor, LPA1, during embryonic development, in particular, vascular formation. Whole-mount in situ hybridization experiments revealed that zebrafish lpa1 (zlpa1) was ubiquitously expressed early in development, and its expression domains were later localized to the head region and the vicinity of the dorsal aorta. The expression of zlpa1 surrounding the dorsal aorta suggests its role in vasculature development. Knocking down of zLPA1 by injecting morpholino (MO) oligonucleotides at 0.625-1.25 ng per embryo resulted in the absence of thoracic duct and edema in pericardial sac and trunk in a dose-dependent manner. These zlpa1-MO-resulted defects could be specifically rescued by ectopic expression of zlpa1. In addition, overexpression of vegf-c, a well-known lymphangiogenic factor, also partially ameliorated the inhibition of thoracic duct development. Taken together, these results demonstrate that LPA1 is necessary for lymphatic vessel formation during embryonic development in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping