PUBLICATION
Pnas4 is a novel regulator for convergence and extension during vertebrate gastrulation
- Authors
- Yao, S., Xie, L., Qian, M., Yang, H., Zhou, L., Zhou, Q., Yan, F., Gou, L., Wei, Y., Zhao, X., and Mo, X.
- ID
- ZDB-PUB-080616-12
- Date
- 2008
- Source
- FEBS letters 582(15): 2325-2332 (Journal)
- Registered Authors
- Mo, Xianming, Yao, Shaohua, Zhao, Xia
- Keywords
- Gastrulation, Convergence and extension, Anterior migration, Pnas4
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cell Movement*/genetics
- Gastrula/cytology*
- Gastrula/metabolism
- Gastrulation*/genetics
- Gene Expression
- Molecular Sequence Data
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 18538138 Full text @ FEBS Lett.
Citation
Yao, S., Xie, L., Qian, M., Yang, H., Zhou, L., Zhou, Q., Yan, F., Gou, L., Wei, Y., Zhao, X., and Mo, X. (2008) Pnas4 is a novel regulator for convergence and extension during vertebrate gastrulation. FEBS letters. 582(15):2325-2332.
Abstract
Recent studies show that human Pnas4 might be tumor associated, while its function remains unknown. Here, we investigate the developmental function of Pnas4 using zebrafish as a model system. Knocking down Pnas4 causes gastrulation defects with a shorter and broader axis, as well as a posteriorly mis-positioned prechordal plate, due to the defective convergence and extension movement. Conversely, over-expression of Pnas4 mRNA leads to an elongated body axis. We further demonstrate that Pnas4 is required cell-autonomously for dorsal convergence but not for anterior migration. In addition, genetic interaction assays indicate that Pnas4 might act in parallel with non-canonical Wnt signal in the regulation of cell movement. Our data suggest that Pnas4 is a key regulator of cell movement during gastrulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping