PUBLICATION
Independent and dynamic reallocation of pitx gene expression during vertebrate evolution, with emphasis on fish pituitary development
- Authors
- Angotzi, A.R., Ersland, K.M., Mungpakdee, S., Stefansson, S., and Chourrout, D.
- ID
- ZDB-PUB-080527-5
- Date
- 2008
- Source
- Gene 417(1-2): 19-26 (Journal)
- Registered Authors
- Chourrout, Daniel
- Keywords
- Pituitary, Development, Genome duplication, Salmon, Fish, Evolution
- MeSH Terms
-
- Animals
- Biological Evolution*
- Fishes/embryology
- Fishes/genetics*
- Gene Duplication
- Gene Expression
- Morphogenesis
- Phylogeny
- Pituitary Gland/embryology*
- Pituitary Gland/metabolism
- Salmon
- Transcription Factors/genetics
- Zebrafish
- Zebrafish Proteins/genetics
- PubMed
- 18486365 Full text @ Gene
Citation
Angotzi, A.R., Ersland, K.M., Mungpakdee, S., Stefansson, S., and Chourrout, D. (2008) Independent and dynamic reallocation of pitx gene expression during vertebrate evolution, with emphasis on fish pituitary development. Gene. 417(1-2):19-26.
Abstract
Several transcription regulators play key roles during pituitary morphogenesis. Well known intrinsic signals of the adenohypophysis such as the K(50)paired-like homeodomain proteins regulate commitment, proliferation, differential specification and maintenance of adenohypophyseal cells. We have cloned and successively characterized the mRNA localization of three pitx gene-pairs and three of their splice variants in salmon, pitx1alpha, pitx1beta; pitx2alpha, pitx2beta; pitx3alpha, pitx3beta; pitx1alphash, pitx1betash and pitx2alphaA. The high level of conservation between the pitx paralog-pairs indicates that they likely arose from lineage-specific genome duplication. We also report the isolation of a pitx1 gene in zebrafish. Comparative ISH studies of zebrafish, salmon and mouse pitx genes indicate both conservation and divergence of spatial expression domains in vertebrates. Significant differences were observed between the expressions of pitx orthologs during pituitary development. We suggest that the ancestral pituitary expression at early and late events of morphogenesis is preserved in different species through complementary shuffling of expression between the distinct pitx members of the family. Moreover, ISH analysis of the pitx salmon repertoire shows rapid evolution in this lineage, differences in spatio-temporal expression are observed between gene duplicates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping