PUBLICATION

Sparc (Osteonectin) functions in morphogenesis of the pharyngeal skeleton and inner ear

Authors
Rotllant, J., Liu, D., Yan, Y.L., Postlethwait, J.H., Westerfield, M., and Du, S.J.
ID
ZDB-PUB-080429-7
Date
2008
Source
Matrix biology : journal of the International Society for Matrix Biology   27(6): 561-572 (Journal)
Registered Authors
Du, Shao Jun (Jim), Liu, Dong, Postlethwait, John H., Rotllant, Josep, Westerfield, Monte, Yan, Yi-Lin
Keywords
Cartilage, Col2a1a, Osteonectin, Otic vesicle, Otx1, Sox9, Sparc
MeSH Terms
  • Animals
  • Body Patterning
  • Ear, Inner/anatomy & histology*
  • Ear, Inner/embryology*
  • Ear, Inner/metabolism
  • Gene Expression Regulation, Developmental
  • Humans
  • In Situ Hybridization
  • Morphogenesis*
  • Oligonucleotides, Antisense/genetics
  • Oligonucleotides, Antisense/metabolism
  • Osteonectin/genetics
  • Osteonectin/metabolism*
  • Pharynx/anatomy & histology*
  • Pharynx/embryology*
  • Pharynx/metabolism
  • Phenotype
  • Skeleton
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 23)
PubMed
18430553 Full text @ Matrix Biol.
Abstract
Sparc (Osteonectin), a matricellular glycoprotein expressed by many differentiated cells, is a major non-collagenous constituent of vertebrate bones. Recent studies indicate that Sparc expression appears early in development, although its function and regulation during embryogenesis are largely unknown. We cloned zebrafish sparc and investigated its role during development, using a mo rpholino antisense oligonucleotide-based knockdown approach. Consistent with its strong expression in the otic vesicle and developing pharyngeal cartilages, knockdown of Sparc function resulted in specific inner ear and cartilage defects that are highlighted by changes in gene expression, morphology and behavior. We rescued the knockdown phenotypes by co-injecting sparc mRNA, providing evidence that the knockdown phenotype is due specifically to impairment of Sparc function. A comparison of the phenotypes of Sparc knockdown and known zebrafish mutants with similar defects places Sparc downstream of sox9 in the genetic network that regulates development of the pharyngeal skeleton and inner ear of vertebrates.
Genes / Markers
Marker Marker Type Name
atp1a1a.4GENEATPase Na+/K+ transporting subunit alpha 1a, tandem duplicate 4
cldnaGENEclaudin a
col2a1aGENEcollagen, type II, alpha 1a
dlx2aGENEdistal-less homeobox 2a
dlx3bGENEdistal-less homeobox 3b
eya1GENEEYA transcriptional coactivator and phosphatase 1
msx3GENEmuscle segment homeobox 3
otx1GENEorthodenticle homeobox 1
sox9aGENESRY-box transcription factor 9a
sox9bGENESRY-box transcription factor 9b
1 - 10 of 12
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Figures
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
AB + MO2-sparcstandard conditionsDay 4
AB + MO2-sparcstandard conditionsDay 4
AB + MO2-sparcstandard conditionsDay 4
AB + MO2-sparcstandard conditionsDay 4
AB + MO2-sparcstandard conditionsDay 4
AB + MO2-sparcstandard conditionsDay 4
AB + MO2-sparcstandard conditionsDay 4
AB + MO2-sparcstandard conditionsDay 4
AB + MO2-sparcstandard conditionsDay 4
AB + MO2-sparcstandard conditionsDays 7-13
1 - 10 of 45
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b971
    		Deficiency
    hi1134TgTransgenic Insertion
    1 - 2 of 2
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    Human Disease / Model
    Sequence Targeting Reagents
    Fish
    Antibodies
    Orthology
    Engineered Foreign Genes
    No data available
    Mapping
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    Citation
    Rotllant, J., Liu, D., Yan, Y.L., Postlethwait, J.H., Westerfield, M., and Du, S.J. (2008) Sparc (Osteonectin) functions in morphogenesis of the pharyngeal skeleton and inner ear. Matrix biology : journal of the International Society for Matrix Biology. 27(6):561-572.