PUBLICATION
Suppression of the endoplasmic reticulum calcium pump during zebrafish gastrulation affects left-right asymmetry of the heart and brain
- Authors
- Kreiling, J.A., Balantac, Z.L., Crawford, A.R., Ren, Y., Toure, J., Zchut, S., Kochilas, L., and Creton, R.
- ID
- ZDB-PUB-080327-14
- Date
- 2008
- Source
- Mechanisms of Development 125(5-6): 396-410 (Journal)
- Registered Authors
- Creton, Robbert
- Keywords
- Calcium, Endoplasmic reticulum, Signaling, Gastrulation, Danio rerio, ER
- MeSH Terms
-
- Animals
- Body Patterning
- Brain/embryology*
- Calcium/metabolism
- Calcium/physiology*
- Dyneins/biosynthesis*
- Dyneins/physiology
- Endoplasmic Reticulum/metabolism*
- Fetal Proteins
- Gastrula/physiology*
- Gene Expression Regulation, Developmental
- Heart/embryology*
- In Situ Hybridization
- Models, Biological
- Sodium-Calcium Exchanger/biosynthesis*
- Sodium-Calcium Exchanger/physiology
- T-Box Domain Proteins/biosynthesis*
- T-Box Domain Proteins/physiology
- Thapsigargin/pharmacology
- Zebrafish
- Zebrafish Proteins/biosynthesis*
- Zebrafish Proteins/physiology
- PubMed
- 18359204 Full text @ Mech. Dev.
Citation
Kreiling, J.A., Balantac, Z.L., Crawford, A.R., Ren, Y., Toure, J., Zchut, S., Kochilas, L., and Creton, R. (2008) Suppression of the endoplasmic reticulum calcium pump during zebrafish gastrulation affects left-right asymmetry of the heart and brain. Mechanisms of Development. 125(5-6):396-410.
Abstract
Vertebrate embryos generate striking Ca(2+) patterns, which are unique regulators of dynamic developmental events. In the present study, we used zebrafish embryos as a model system to examine the developmental roles of Ca(2+) during gastrulation. We found that gastrula stage embryos maintain a distinct pattern of cytosolic Ca(2+) along the dorsal-ventral axis, with higher Ca(2+) concentrations in the ventral margin and lower Ca(2+) concentrations in the dorsal margin and dorsal forerunner cells. Suppression of the endoplasmic reticulum Ca(2+) pump with 0.5muM thapsigargin elevates cytosolic Ca(2+) in all embryonic regions and induces a randomization of laterality in the heart and brain. Affected hearts, visualorsal forerunner cells. Suppression of the endoplasmic reticulum Ca(2+) pump with 0.5muM thapsigargin elevates cytosolic Ca(2+) in all emized in living embryos by a subtractive imaging technique, displayed either a reversal or loss of left-right asymmetry. Brain defects include a left-right reversal of pitx2 expression in the dorsal diencephalon and a left-right reversal of the prominent habenular nucleus in the brain. Embryos are sensitive to inhibition of the endoplasmic reticulum Ca(2+) pump during early and mid gastrulation and lose their sensitivity during late gastrulation and early segmentation. Suppression of the endoplasmic reticulum Ca(2+) pump during gastrulation inhibits expression of no tail (ntl) and left-right dynein related (lrdr) in the dorsal forerunner cells and affects development of Kupffer's vesicle, a ciliated organ that generates a counter-clockwise flow of fluid. Previous studies have shown that Ca(2+) plays a role in Kupffer's vesicle function, influencing ciliary motility and translating the vesicle's counter-clockwise flow into asymmetric patterns of gene expression. The present results suggest that Ca(2+) plays an additional role in the formation of Kupffer's vesicle.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping