PUBLICATION
Control of chemokine-guided cell migration by ligand sequestration
- Authors
- Boldajipour, B., Mahabaleshwar, H., Kardash, E., Reichman-Fried, M., Blaser, H., Minina, S., Wilson, D., Xu, Q., and Raz, E.
- ID
- ZDB-PUB-080309-8
- Date
- 2008
- Source
- Cell 132(3): 463-473 (Journal)
- Registered Authors
- Blaser, Heiko, Boldajipour, Bijan, Kardash, Elena, Mahabaleshwar, Harsha, Minina, Sofia, Raz, Erez, Reichman-Fried, Michal, Xu, Qiling
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Movement*
- Cell Polarity
- Chemokine CXCL12/metabolism*
- Embryo, Nonmammalian/cytology
- Gene Expression Regulation, Developmental
- Germ Cells/cytology*
- Receptors, CXCR/genetics
- Receptors, CXCR/metabolism*
- Zebrafish/embryology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 18267076 Full text @ Cell
Citation
Boldajipour, B., Mahabaleshwar, H., Kardash, E., Reichman-Fried, M., Blaser, H., Minina, S., Wilson, D., Xu, Q., and Raz, E. (2008) Control of chemokine-guided cell migration by ligand sequestration. Cell. 132(3):463-473.
Abstract
Primordial germ cell (PGC) migration in zebrafish is directed by the chemokine SDF-1a that activates its receptor CXCR4b. Little is known about the molecular mechanisms controlling the distribution of this chemoattractant in vivo. We demonstrate that the activity of a second SDF-1/CXCL12 receptor, CXCR7, is crucial for proper migration of PGCs toward their targets. We show that CXCR7 functions primarily in the somatic environment rather than within the migrating cells. In CXCR7 knocked-down embryos, the PGCs exhibit a phenotype that signifies defects in SDF-1a gradient formation as the cells fail to polarize effectively and to migrate toward their targets. Indeed, somatic cells expressing CXCR7 show enhanced internalization of the chemokine suggesting that CXCR7 acts as a sink for SDF-1a, thus allowing the dynamic changes in the transcription of sdf-1a to be mirrored by similar dynamics at the protein level.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping