PUBLICATION
PTRF-Cavin, a Conserved Cytoplasmic Protein Required for Caveola Formation and Function
- Authors
- Hill, M.M., Bastiani, M., Luetterforst, R., Kirkham, M., Kirkham, A., Nixon, S.J., Walser, P., Abankwa, D., Oorschot, V.M., Martin, S., Hancock, J.F., and Parton, R.G.
- ID
- ZDB-PUB-080209-9
- Date
- 2008
- Source
- Cell 132(1): 113-124 (Journal)
- Registered Authors
- Parton, Robert G.
- Keywords
- none
- MeSH Terms
-
- Animals
- Bees
- Caveolae/metabolism*
- Caveolae/ultrastructure
- Caveolin 1/metabolism
- Cell Line
- Cell Line, Tumor
- Cell Membrane/metabolism*
- Cell Membrane/ultrastructure
- Cells, Cultured
- Conserved Sequence
- Cricetinae
- Cytoplasm/metabolism*
- Cytoplasm/ultrastructure
- Evolution, Molecular
- Fibroblasts
- Golgi Apparatus/metabolism
- Golgi Apparatus/ultrastructure
- Intracellular Membranes/metabolism
- Intracellular Membranes/ultrastructure
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- Mice
- Mice, Knockout
- Molecular Sequence Data
- NIH 3T3 Cells
- Notochord/embryology
- Notochord/metabolism
- Notochord/ultrastructure
- RNA-Binding Proteins
- Species Specificity
- Zebrafish
- PubMed
- 18191225 Full text @ Cell
Citation
Hill, M.M., Bastiani, M., Luetterforst, R., Kirkham, M., Kirkham, A., Nixon, S.J., Walser, P., Abankwa, D., Oorschot, V.M., Martin, S., Hancock, J.F., and Parton, R.G. (2008) PTRF-Cavin, a Conserved Cytoplasmic Protein Required for Caveola Formation and Function. Cell. 132(1):113-124.
Abstract
Caveolae are abundant cell-surface organelles involved in lipid regulation and endocytosis. We used comparative proteomics to identify PTRF (also called Cav-p60, Cavin) as a putative caveolar coat protein. PTRF-Cavin selectively associates with mature caveolae at the plasma membrane but not Golgi-localized caveolin. In prostate cancer PC3 cells, and during development of zebrafish notochord, lack of PTRF-Cavin expression correlates with lack of caveolae, and caveolin resides on flat plasma membrane. Expression of PTRF-Cavin in PC3 cells is sufficient to cause formation of caveolae. Knockdown of PTRF-Cavin reduces caveolae density, both in mammalian cells and in the zebrafish. Caveolin remains on the plasma membrane in PTRF-Cavin knockdown cells but exhibits increased lateral mobility and accelerated lysosomal degradation. We conclude that PTRF-Cavin is required for caveola formation and sequestration of mobile caveolin into immobile caveolae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping