PUBLICATION

Members of the miRNA-200 Family Regulate Olfactory Neurogenesis

Authors

Choi, P.S., Zakhary, L., Choi, W.Y., Caron, S., Alvarez-Saavedra, E., Miska, E.A., McManus, M., Harfe, B., Giraldez, A.J., Horvitz, R.H., Schier, A.F., and Dulac, C.

ID

ZDB-PUB-080209-4

Date

2008

Source

Neuron 57(1): 41-55 (Journal)

Registered Authors

Caron, Sophie, Choi, Wen-Yee, Giraldez, Antonio, Schier, Alexander

Keywords

none

MeSH Terms

Age Factors
Animals
Cell Differentiation/physiology*
Cell Proliferation*
Embryo, Nonmammalian
Embryonic Stem Cells/physiology
Gene Expression Profiling
Gene Expression Regulation, Developmental/physiology
Mice
MicroRNAs/classification
MicroRNAs/physiology*
Nerve Tissue Proteins/genetics
Nerve Tissue Proteins/metabolism
Neurons/physiology*
Olfactory Pathways/cytology*
Olfactory Pathways/physiology
Oligonucleotide Array Sequence Analysis
Zebrafish

(all 18)

PubMed

18184563 Full text @ Neuron

Abstract

MicroRNAs (miRNAs) are highly expressed in vertebrate neural tissues, but the contribution of specific miRNAs to the development and function of different neuronal populations is still largely unknown. We report that miRNAs are required for terminal differentiation of olfactory precursors in both mouse and zebrafish but are dispensable for proper function of mature olfactory neurons. The repertoire of miRNAs expressed in olfactory tissues contains over 100 distinct miRNAs. A subset, including the miR-200 family, shows high olfactory enrichment and expression patterns consistent with a role during olfactory neurogenesis. Loss of function of the miR-200 family phenocopies the terminal differentiation defect observed in absence of all miRNA activity in olfactory progenitors. Our data support the notion that vertebrate tissue differentiation is controlled by conserved subsets of organ-specific miRNAs in both mouse and zebrafish and provide insights into control mechanisms underlying olfactory differentiation in vertebrates.

Genes / Markers

Marker	Marker Type	Name
ascl1a	GENE	achaete-scute family bHLH transcription factor 1a
dicer1	GENE	dicer 1, ribonuclease type III
dnd1	GENE	DND microRNA-mediated repression inhibitor 1
dre-mir-429	MIRNAG	microRNA 429
foxg1a	GENE	forkhead box G1a
lfng	GENE	LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
mir141	MIRNAG	microRNA 141
mir200a	MIRNAG	microRNA 200a
mir200b	MIRNAG	microRNA 200b
mir200c	MIRNAG	microRNA 200c

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Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
ascl1a	WT	standard conditions	Long-pec		brain	ISH	Fig. 7
ascl1a	WT + MO1-dre-mir-429 + MO1-mir141,mir200a + MO1-mir200b,mir200c	standard conditions	Long-pec		brain	ISH	Fig. 7
dre-mir-429	WT	standard conditions	Long-pec		peripheral olfactory organ	ISH	Fig. 7
dre-mir-429	WT	standard conditions	Long-pec		peripheral olfactory organ	ISH	Fig. S4
dre-mir-429	WT + MO1-dre-mir-429	standard conditions	Long-pec	Not Detected	peripheral olfactory organ	ISH	Fig. S4
dre-mir-429	WT + MO1-dre-mir-429 + MO1-mir141,mir200a + MO1-mir200b,mir200c	standard conditions	Long-pec	Not Detected	peripheral olfactory organ	ISH	Fig. 7
dre-mir-429	WT + MO1-mir141,mir200a	standard conditions	Long-pec		peripheral olfactory organ	ISH	Fig. S4
dre-mir-429	WT + MO1-mir200b,mir200c	standard conditions	Long-pec		peripheral olfactory organ	ISH	Fig. S4
foxg1a	dicer1^hu715/hu715	chemical treatment: pharmaceutical	Long-pec		peripheral olfactory organ	ISH	Fig. 7
foxg1a	WT	standard conditions	Long-pec		brain	ISH	Fig. 7

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-dre-mir-429 + MO1-mir141,mir200a + MO1-mir200b,mir200c	standard conditions	Long-pec	nose development arrested, abnormal	Fig. 7
WT + MO1-dre-mir-429 + MO1-mir141,mir200a + MO1-mir200b,mir200c	standard conditions	Long-pec	olfactory epithelium immature, abnormal	Fig. 7
dicer1^hu715/hu715	chemical treatment: pharmaceutical	Long-pec	nose development arrested, abnormal	Fig. 7
dicer1^hu715/hu715	chemical treatment: pharmaceutical	Long-pec	olfactory epithelium immature, abnormal	Fig. 7
dicer1^hu896/hu896	chemical treatment: pharmaceutical	Long-pec	nose development arrested, abnormal	Fig. 7
dicer1^hu896/hu896	chemical treatment: pharmaceutical	Long-pec	olfactory epithelium immature, abnormal	Fig. 7

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
hu715		Point Mutation	dicer1
hu896		Point Mutation	dicer1

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
dre-mir-429	MO1-dre-mir-429	MRPHLNO
mir141	MO1-mir141,mir200a	MRPHLNO
mir200a	MO1-mir141,mir200a	MRPHLNO
mir200a	MO1-mir200a	MRPHLNO
mir200b	MO1-mir200b,mir200c	MRPHLNO
mir200c	MO1-mir200b,mir200c	MRPHLNO

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Fish

Fish
dicer1^hu715/hu715
dicer1^hu896/hu896
WT
WT + MO1-dre-mir-429
WT + MO1-dre-mir-429 + MO1-mir141,mir200a + MO1-mir200b,mir200c
WT + MO1-mir141,mir200a
WT + MO1-mir200b,mir200c

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Choi et al., 2008 ZDB-PUB-080209-4 PMID:18184563

Members of the miRNA-200 Family Regulate Olfactory Neurogenesis

Choi et al., 2008

ZDB-PUB-080209-4
PMID:18184563