PUBLICATION

Combined activity of the two Gli2 genes of zebrafish play a major role in Hedgehog signaling during zebrafish neurodevelopment

Authors

Ke, Z., Kondrichin, I., Gong, Z., and Korzh, V.

ID

ZDB-PUB-071210-37

Date

2008

Source

Molecular and cellular neurosciences 37(2): 388-401 (Journal)

Registered Authors

Gong, Zhiyuan, Ke, Zhiyuan, Kondrychyn, Igor, Korzh, Vladimir

Keywords

Gene duplication, Gli2b, Neural progenitors, Hindbrain, Rhombomere 4, Motoneurons, Oligodendrocytes

MeSH Terms

Animals
Basic Helix-Loop-Helix Transcription Factors/metabolism
Branchial Region/embryology
Branchial Region/innervation
Cell Differentiation/physiology
Cell Proliferation
Gene Expression Regulation, Developmental/genetics*
Hedgehog Proteins/genetics
Homeodomain Proteins/metabolism
Nerve Tissue Proteins/metabolism
Nervous System/cytology
Nervous System/embryology*
Nervous System/metabolism
Neural Tube/cytology
Neural Tube/embryology
Neural Tube/metabolism
Neurons/cytology
Neurons/metabolism*
Oligonucleotides, Antisense/pharmacology
Protein Isoforms/genetics
Receptor, Notch1/metabolism
Rhombencephalon/cytology
Rhombencephalon/embryology
Rhombencephalon/metabolism
Signal Transduction/genetics
Transcription Factors/genetics*
Zebrafish/embryology*
Zebrafish/genetics*
Zebrafish/metabolism
Zebrafish Proteins/genetics*
Zebrafish Proteins/metabolism

(all 31)

PubMed

18060804 Full text @ Mol. Cell Neurosci.

Abstract

It has been proposed that the downstream mediator of the evolutionarily conserved Hedgehog pathway Gli2 plays a relatively minor role in neural development of zebrafish. The second gli2 of zebrafish, gli2b, is expressed in the neural plate and the central nervous system. Our comparative analysis of the developmental role of gli2/gli2b demonstrate a major role of the two Gli2s in mediating Hh signaling. The Gli2s play an early Hh-independent repressor role in the maintenance of neural progenitors and an Hh-dependent activating role during cell differentiation in the floor plate, branchial motor neurons, and sensory neurons. Our analysis of Gli2b loss-of-function using antisense morpholino oligonucleotides indicates that the functions of the two Gli2s diverged in evolution. Gli2b acts in cell proliferation and plays an early role in the hindbrain within a regulatory cascade involving Notch and Ngn1, as well as a role as specific activator in rhombomere 4.

Genes / Markers

Marker	Marker Type	Name
alcama	GENE	activated leukocyte cell adhesion molecule a
bmp2a	GENE	bone morphogenetic protein 2a
bmp2b	GENE	bone morphogenetic protein 2b
bmp4	GENE	bone morphogenetic protein 4
dnajc2	GENE	DnaJ (Hsp40) homolog, subfamily C, member 2
egr2b	GENE	early growth response 2b
elavl3	GENE	ELAV like neuron-specific RNA binding protein 3
gfap	GENE	glial fibrillary acidic protein
gli2a	GENE	GLI family zinc finger 2a
gli2b	GENE	GLI family zinc finger 2b

1 - 10 of 23

Show

Figures

Show all Figures

Expression

Gene	Antibody	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
alcama	zn-5	WT	control	Long-pec		abducens motor nucleus	IHC	Fig. 5
alcama	zn-5	WT	control	Long-pec		secondary motor neuron	IHC	Fig. 5
alcama	zn-5	WT + MO1-gli2b	standard conditions	Long-pec	Not Detected	abducens motor nucleus	IHC	Fig. 5
alcama	zn-5	WT + MO1-gli2b	standard conditions	Long-pec	Not Detected	secondary motor neuron	IHC	Fig. 5
bmp2a		WT	standard conditions	Prim-5		whole organism	RTPCR	Fig. 7
bmp2a		WT + MO1-gli2b + MO2-gli2a	standard conditions	Prim-5		whole organism	RTPCR	Fig. 7
bmp2b		WT	standard conditions	Prim-5		whole organism	RTPCR	Fig. 7
bmp2b		WT + MO1-gli2b + MO2-gli2a	standard conditions	Prim-5		whole organism	RTPCR	Fig. 7
bmp4		WT	standard conditions	Prim-5		whole organism	RTPCR	Fig. 7
bmp4		WT + MO1-gli2b + MO2-gli2a	standard conditions	Prim-5		whole organism	RTPCR	Fig. 7

1 - 10 of 83

Show

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-gli2b	standard conditions	Prim-5	oligodendrocyte differentiation disrupted, abnormal	Fig. 4
WT + MO1-gli2b	standard conditions	Prim-15	floor plate formation disrupted, abnormal	Fig. 6
WT + MO1-gli2b	standard conditions	Prim-15	floor plate rhombomere region increased width, abnormal	Fig. 6
WT + MO1-gli2b	standard conditions	Prim-15	fourth ventricle increased size, abnormal	text only
WT + MO1-gli2b	standard conditions	Prim-15	hindbrain astrocyte decreased amount, abnormal	Fig. 1
WT + MO1-gli2b	standard conditions	Prim-15	horizontal myoseptum morphology, normal	Fig. S1
WT + MO1-gli2b	standard conditions	Long-pec	abducens motor nucleus aplastic, abnormal	Fig. 5
WT + MO1-gli2b	standard conditions	Long-pec	axonogenesis disrupted, abnormal	Fig. S2
WT + MO1-gli2b	standard conditions	Long-pec	brain decreased size, abnormal	text only
WT + MO1-gli2b	standard conditions	Long-pec	facial nerve motor nucleus mislocalised laterally, abnormal	Fig. 5

1 - 10 of 26

Show

Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
b641		Point Mutation	smo
rw0Tg	Tg(isl1a:GFP)	Transgenic Insertion
sqet3Et	Et(krt4:EGFP)	Transgenic Insertion
ty17a		Point Mutation	gli2a

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
gli2a	MO2-gli2a	MRPHLNO
gli2b	MO1-gli2b	MRPHLNO
gli2b	MO2-gli2b	MRPHLNO

Fish

Fish
gli2a^ty17a/ty17a
gli2a^ty17a/ty17a + MO1-gli2b
rw0Tg
rw0Tg + MO1-gli2b
smo^b641/b641
smo^b641/b641 + MO1-gli2b
sqet3Et
sqet3Et + MO1-gli2b
WT
WT + MO1-gli2b

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP
GFP	EFG	GFP

Mapping

Ke et al., 2008 ZDB-PUB-071210-37 PMID:18060804

Combined activity of the two Gli2 genes of zebrafish play a major role in Hedgehog signaling during zebrafish neurodevelopment

Ke et al., 2008

ZDB-PUB-071210-37
PMID:18060804