PUBLICATION

Cadherin-mediated adhesion regulates posterior body formation

Authors

Harrington, M.J., Hong, E., Fasanmi, O., and Brewster, R.

ID

ZDB-PUB-071210-3

Date

2007

Source

BMC Developmental Biology 7(1): 130 (Journal)

Registered Authors

Brewster, Rachel, Harrington, Michael, Hong, Elim

Keywords

none

MeSH Terms

Animals
Apoptosis
Body Patterning/genetics*
Cadherins/genetics*
Cell Adhesion
Cell Polarity
Embryo, Nonmammalian/embryology*
Gene Expression Regulation, Developmental
In Situ Hybridization
Oligonucleotides, Antisense
Protein Biosynthesis
Tail/embryology
Zebrafish/embryology*
Zebrafish/genetics
Zebrafish Proteins/genetics*

(all 15)

PubMed

18045497 Full text @ BMC Dev. Biol.

Abstract

BACKGROUND: The anterior-posterior axis of the vertebrate embryo undergoes a dramatic elongation during early development. Convergence and extension of the mesoderm, occurring during gastrulation, initiates the narrowing and lengthening of the embryo. However the lengthening of the axis continues during post-gastrula stages in the tailbud region, and is thought to involve convergent extension movements as well as other cell behaviors specific to posterior regions. RESULTS: We demonstrate here, using a semi-dominant N-cadherin allele, that members of the classical cadherin subfamily of cell-cell adhesion molecules are required for tailbud elongation in the zebrafish. In vivo imaging of cell behaviors suggests that the extension of posterior axial mesodermal cells is impaired in embryos that carry the semi-dominant N-cadherin allele. This defect most likely results from a general loss of cell-cell adhesion in the tailbud region. Consistent with these observations, N-cadherin is expressed throughout the tailbud during post-gastrulation stages. In addition, we show that N-cadherin interacts synergistically with Strabismus, a member of the non-canonical Wnt signaling/planar cell polarity pathway, to mediate tail morphogenesis. CONCLUSIONS: We provide the first evidence here that N-cadherin and other members of the classical cadherin subfamily function in parallel with the planar cell polarity pathway to shape the posterior axis during post-gastrulation stages. These findings further highlight the central role that adhesion molecules play in the cellular rearrangements that drive morphogenesis in vertebrates and identify classical cadherins as major contributors to tail development.

Genes / Markers

Marker	Marker Type	Name
cdh2	GENE	cadherin 2, type 1, N-cadherin (neuronal)
dlx3b	GENE	distal-less homeobox 3b
gpc4	GENE	glypican 4
myod1	GENE	myogenic differentiation 1
pcdh8	GENE	protocadherin 8
sox3	GENE	SRY-box transcription factor 3
tbxta	GENE	T-box transcription factor Ta
vangl2	GENE	VANGL planar cell polarity protein 2

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Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-cdh2	standard conditions	Prim-15	posterior neural tube T-shaped, abnormal	Fig. S1
WT + MO1-vangl2	standard conditions	1-4 somites	neural plate width, normal	Fig. 10
WT + MO1-vangl2	standard conditions	1-4 somites	notochord width, normal	Fig. 10
WT + MO1-vangl2	standard conditions	5-9 somites	paraxial mesoderm increased width, abnormal	Fig. 10
WT + MO1-vangl2	standard conditions	5-9 somites	somite increased width, abnormal	Fig. 10
WT + MO1-vangl2	standard conditions	Prim-15	post-vent region morphology, normal	Fig. 8
cdh2^m117/+	standard conditions	5-9 somites	convergent extension involved in neural plate elongation disrupted, abnormal	Fig. 3
cdh2^m117/+	standard conditions	5-9 somites	neural rod shape, abnormal	Fig. 3
cdh2^m117/+	standard conditions	Prim-15	post-vent region length, normal	Fig. 2
cdh2^m117/+; cdh2^p79emcf/+	standard conditions	Prim-15	brain morphology, abnormal	Fig. 1

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
hi1688Tg	Tg(nLacz-GTvirus)	Transgenic Insertion	gpc4
m117		Point Mutation	cdh2
m747		Point Mutation	vangl2
p79emcf		Point Mutation	cdh2
r210		Point Mutation	cdh2

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
cdh2	MO1-cdh2	MRPHLNO
vangl2	MO1-vangl2	MRPHLNO

Fish

Fish
cdh2^m117/m117
cdh2^m117/m117 + MO1-vangl2
cdh2^m117/+
cdh2^m117/+
cdh2^m117/+; cdh2^p79emcf/+
cdh2^{p79emcf/p79emcf}
cdh2^{p79emcf/p79emcf} + MO1-vangl2
cdh2^r210/r210
gpc4^{hi1688Tg/hi1688Tg}
vangl2^m747/m747

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Antibodies

Orthology

Engineered Foreign Genes

Mapping

Harrington et al., 2007 ZDB-PUB-071210-3 PMID:18045497

Cadherin-mediated adhesion regulates posterior body formation

Harrington et al., 2007

ZDB-PUB-071210-3
PMID:18045497