PUBLICATION
Immunoglobulin light chain (IgL) genes in zebrafish: Genomic configurations and inversional rearrangements between (V(L)-J(L)-C(L)) gene clusters
- Authors
- Zimmerman, A.M., Yeo, G., Howe, K., Maddox, B.J., and Steiner, L.A.
- ID
- ZDB-PUB-071125-19
- Date
- 2008
- Source
- Developmental and comparative immunology 32(4): 421-434 (Journal)
- Registered Authors
- Howe (fka Jekosch), Kerstin, Steiner, Lisa, Zimmerman, Ana
- Keywords
- Immunoglobulin, Zebrafish, Rearrangement, Genome, RSS
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Chromosomes/genetics
- Gene Rearrangement, B-Lymphocyte, Light Chain*
- Genes, Immunoglobulin Light Chain*
- Immunoglobulin Constant Regions/genetics
- Immunoglobulin Joining Region/genetics
- Immunoglobulin Light Chains/chemistry
- Immunoglobulin Light Chains/genetics*
- Immunoglobulin Light Chains/immunology
- Immunoglobulin Variable Region/genetics
- Molecular Sequence Data
- Multigene Family
- Phylogeny
- Sequence Alignment
- Sequence Analysis, DNA
- Zebrafish/genetics*
- Zebrafish/immunology*
- PubMed
- 18022691 Full text @ Dev. Comp. Immunol.
Citation
Zimmerman, A.M., Yeo, G., Howe, K., Maddox, B.J., and Steiner, L.A. (2008) Immunoglobulin light chain (IgL) genes in zebrafish: Genomic configurations and inversional rearrangements between (V(L)-J(L)-C(L)) gene clusters. Developmental and comparative immunology. 32(4):421-434.
Abstract
In mammals, Immunoglobulin light chain (IgL) are localized to two chromosomal regions (designated kappa and lambda). Here we report a genome-wide survey of IgL genes in the zebrafish revealing (V(L)-J(L)-C(L)) clusters spanning 5 separate chromosomes. To elucidate IgL loci present in the zebrafish genome assembly (Zv6), conventional sequence similarity searches and a novel scanning approach based on recombination signal sequence (RSS) motifs were applied. RT-PCR with zebrafish cDNA was used to confirm annotations, evaluate VJ-rearrangement possibilities and show that each chromosomal locus is expressed. In contrast to other vertebrates in which IgL exon usage has been studied, inversional rearrangement between (V(L)-J(L)-C(L)) clusters were found. Inter-cluster rearrangements may convey a selective advantage for editing self-reactive receptors and poise zebrafish by virtue of their extensive numbers of V(L), J(L) and C(L) to have greater potential for immunoglobulin gene shuffling than traditionally studied mice and human models.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping