PUBLICATION

Slit1a inhibits retinal ganglion cell arborization and synaptogenesis via Robo2-dependent and -independent pathways

Authors
Campbell, D.S., Stringham, S.A., Timm, A., Xiao, T., Law, M.Y., Baier, H., Nonet, M.L., and Chien, C.B.
ID
ZDB-PUB-071118-3
Date
2007
Source
Neuron   55(2): 231-245 (Journal)
Registered Authors
Baier, Herwig, Campbell, Douglas, Chien, Chi-Bin, Law, Mei-Yee, Nonet, Michael, Xiao, Tong
Keywords
none
MeSH Terms
  • Animals
  • Dendrites/metabolism*
  • Nerve Tissue Proteins/metabolism*
  • Receptors, Immunologic/metabolism*
  • Retinal Ganglion Cells/cytology
  • Retinal Ganglion Cells/metabolism*
  • Superior Colliculi/cytology
  • Superior Colliculi/metabolism
  • Synapses/metabolism*
  • Visual Pathways/cytology
  • Visual Pathways/metabolism*
  • Zebrafish
  • Zebrafish Proteins/metabolism*
(all 13)
PubMed
17640525 Full text @ Neuron
Abstract
Upon arriving at their targets, developing axons cease pathfinding and begin instead to arborize and form synapses. To test whether CNS arborization and synaptogenesis are controlled by Slit-Robo signaling, we followed single retinal ganglion cell (RGC) arbors over time. ast (robo2) mutant and slit1a morphant arbors had more branch tips and greater arbor area and complexity compared to wild-type and concomitantly more presumptive presynaptic sites labeled with YFP-Rab3. Increased arborization in ast was phenocopied by dominant-negative Robo2 expressed in single RGCs and rescued by full-length Robo2, indicating that Robo2 acts cell-autonomously. Time-lapse imaging revealed that ast and slit1a morphant arbors stabilized earlier than wild-type, suggesting a role for Slit-Robo signaling in preventing arbor maturation. Genetic analysis showed that Slit1a acts both through Robo2 and Robo2-independent mechanisms. Unlike previous PNS studies showing that Slits promote branching, our results show that Slits inhibit arborization and synaptogenesis in the CNS.
Genes / Markers
Figures
Figure Gallery (3 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
nm1TgTransgenic Insertion
    te284
      		Point Mutation
      ti272z
        		Point Mutation
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        slit1aMO1-slit1aMRPHLNO
        slit1aMO2-slit1aMRPHLNO
        1 - 2 of 2
        Show
        Fish
        Fish
        nm1Tg
        robo2te284/te284
        robo2ti272z/ti272z
        WT
        WT + MO1-slit1a
        1 - 5 of 5
        Show
        Antibodies
        Orthology
        Engineered Foreign Genes
        Marker Marker Type Name
        YFPEFGYFP
        1 - 1 of 1
        Show
        Mapping
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        Citation
        Campbell, D.S., Stringham, S.A., Timm, A., Xiao, T., Law, M.Y., Baier, H., Nonet, M.L., and Chien, C.B. (2007) Slit1a inhibits retinal ganglion cell arborization and synaptogenesis via Robo2-dependent and -independent pathways. Neuron. 55(2):231-245.