PUBLICATION

Ethanol and acetaldehyde alter NTPDase and 5'-nucleotidase from zebrafish brain membranes

Authors
Rico, E.P., Rosemberg, D.B., Senger, M.R., Arizi, M.D., Dias, R.D., Souto, A.A., Bogo, M.R., and Bonan, C.D.
ID
ZDB-PUB-070820-6
Date
2008
Source
Neurochemistry international   52(1-2): 290-296 (Journal)
Registered Authors
Bonan, Carla Denise
Keywords
Ethanol, Nucleotidase, NTPDase, 5'-Nucleotidase, Adenosine, Zebrafish
MeSH Terms
  • 5'-Nucleotidase/metabolism*
  • Acetaldehyde/pharmacology*
  • Animals
  • Base Sequence
  • Brain/drug effects*
  • Brain/enzymology
  • DNA Primers
  • Ethanol/pharmacology*
  • Pyrophosphatases/metabolism*
  • Zebrafish
PubMed
17698255 Full text @ Neurochem. Int.
CTD
17698255
Abstract
Alcohol abuse is an acute health problem throughout the world and alcohol consumption is linked to the occurrence of several pathological conditions. Here we tested the acute effects of ethanol on NTPDases (nucleoside triphosphate diphosphohydrolases) and 5'-nucleotidase in zebrafish (Danio rerio) brain membranes. The results have shown a decrease on ATP (36.3 and 18.4%) and ADP (30 and 20%) hydrolysis after 0.5 and 1% (v/v) ethanol exposure during 60min, respectively. In contrast, no changes on 5'-nucleotidase activity were observed in zebrafish brain membranes. Ethanol in vitro did not alter ATP and ADP hydrolysis, but AMP hydrolysis was inhibited at 0.5, and 1% (23 and 28%, respectively). Acetaldehyde in vitro, in the range 0.5-1%, inhibited ATP (40-85%) and ADP (28-65%) hydrolysis, whereas AMP hydrolysis was reduced (52, 58 and 64%) at 0.25, 0.5 and 1%, respectively. Acetate in vitro did not alter these enzyme activities. Semi-quantitative expression analysis of NTPDase and 5'-nucleotidase were performed. Ethanol treatment reduced NTPDase1 and three isoforms of NTPDase2 mRNA levels. These findings demonstrate that acute ethanol intoxication may influence the enzyme pathway involved in the degradation of ATP to adenosine, which could affect the responses mediated by adenine nucleotides and nucleosides in zebrafish central nervous system.
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