PUBLICATION
New statistical methods enhance imaging of cameleon fluorescence resonance energy transfer in cultured zebrafish spinal neurons
- Authors
- Fan, X., Majumder, A., Reagin, S.S., Porter, E.L., Sornborger, A.T., Keith, C.H., and Lauderdale, J.D.
- ID
- ZDB-PUB-070711-37
- Date
- 2007
- Source
- Journal of Biomedical Optics 12(3): 034017 (Journal)
- Registered Authors
- Fan, Xiang, Lauderdale, James D.
- Keywords
- none
- MeSH Terms
-
- Algorithms
- Animals
- Calcium/metabolism*
- Calcium Signaling/physiology
- Calcium-Binding Proteins*
- Cells, Cultured
- Data Interpretation, Statistical
- Fluorescence Resonance Energy Transfer/methods*
- Fluorescent Dyes*
- Neurons/cytology
- Neurons/metabolism*
- Recombinant Fusion Proteins*
- Zebrafish/anatomy & histology
- Zebrafish/metabolism*
- PubMed
- 17614725 Full text @ J. Biomed. Opt.
Citation
Fan, X., Majumder, A., Reagin, S.S., Porter, E.L., Sornborger, A.T., Keith, C.H., and Lauderdale, J.D. (2007) New statistical methods enhance imaging of cameleon fluorescence resonance energy transfer in cultured zebrafish spinal neurons. Journal of Biomedical Optics. 12(3):034017.
Abstract
Cameleons are genetically encoded fluorescence resonance energy transfer (FRET)-based Ca(2+) indicators. Attempts to use cameleons to detect neural activity in vertebrate systems have been largely frustrated by the small FRET signal, in contradistinction to the higher signals seen in Drosophila and Caenorhabditis elegans. We have developed a statistical optimization method capable of detecting small ratiometric signals in noisy imaging data, called statistical optimization for the analysis of ratiometric signals. Using this method, we can detect and estimate anticorrelated ratiometric signals with subcellular resolution in cultured, dissociated zebrafish spinal neurons expressing cameleon or loaded with fluo-4 and fura-red. This method may make it possible to use yellow cameleons for measuring neural activity at high resolution in transgenic animals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping