PUBLICATION
Tip-1 induces filopodia growth and is important for gastrulation movements during zebrafish development
- Authors
- Besser, J., Leito, J.T., van der Meer, D.L., and Bagowski, C.P.
- ID
- ZDB-PUB-070409-6
- Date
- 2007
- Source
- Development, growth & differentiation 49(3): 205-214 (Journal)
- Registered Authors
- Bagowski, Christoph P.
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- Embryo, Nonmammalian/chemistry
- Embryo, Nonmammalian/metabolism
- Gastrula/physiology*
- Green Fluorescent Proteins/analysis
- Green Fluorescent Proteins/genetics
- Homeodomain Proteins/antagonists & inhibitors
- Homeodomain Proteins/genetics
- Homeodomain Proteins/physiology*
- Molecular Sequence Data
- Pseudopodia/genetics
- Pseudopodia/physiology*
- RNA, Messenger/analysis
- RNA, Messenger/metabolism
- Recombinant Fusion Proteins/analysis
- Recombinant Fusion Proteins/genetics
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- PubMed
- 17394599 Full text @ Dev. Growth Diff.
Citation
Besser, J., Leito, J.T., van der Meer, D.L., and Bagowski, C.P. (2007) Tip-1 induces filopodia growth and is important for gastrulation movements during zebrafish development. Development, growth & differentiation. 49(3):205-214.
Abstract
Wnt signaling is essential during animal development and also plays important roles in pathological conditions. Two mayor pathways have been described: the beta-catenin-dependent canonical (or classical) pathway and the beta-catenin-independent non-canonical Wnt pathway. Recent binding studies suggest links between the small PDZ protein TIP-1 (Tax-1 interacting protein) to components of both Wnt pathways. We have cloned and characterized the zebrafish tip-1 gene. Whole mount in situ hybridization and semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) indicated that zebrafish tip-1 is present as a maternal RNA and is ubiquitously expressed during early development. After 24 h of development, tip-1 expression was high in the central nervous system (CNS) whereas only weak expression was detected in the caudal regions of the zebrafish embryo. Tip-1 knockdown using antisense morpholino oligonucleotides, as well as ectopic tip-1 expression, led to elongation defects in zebrafish embryos and larvae. Both knockdown and overexpression of tip-1 resulted in a widened goosecoid (gsc) expression domain in shield stage embryos, led to an abbreviated prechordal plate, and to reduced convergent extension movements during gastrulation. We constructed a green fluorescence protein (GFP)/TIP-1 fusion protein which, when expressed in cultured fibroblasts (ZF4-cells), induced filopodia growth. Our observations indicate a role for TIP-1 in gastrulation movements and in filopodia growth induction.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping