PUBLICATION
Targeting neural circuitry in zebrafish using GAL4 enhancer trapping
- Authors
- Scott, E.K., Mason, L., Arrenberg, A.B., Ziv, L., Gosse, N.J., Xiao, T., Chi, N.C., Asakawa, K., Kawakami, K., and Baier, H.
- ID
- ZDB-PUB-070330-18
- Date
- 2007
- Source
- Nature Methods 4(4): 323-326 (Journal)
- Registered Authors
- Arrenberg, Aristides, Baier, Herwig, Gosse, Nathan, Kawakami, Koichi, Mason, Lindsay, Scott, Ethan, Xiao, Tong, Ziv, Limor
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Brain*/cytology
- Brain*/embryology
- Brain*/metabolism
- DNA-Binding Proteins
- Enhancer Elements, Genetic*
- Gene Expression Regulation, Developmental*
- Humans
- Neurons*/metabolism
- Pilot Projects
- Saccharomyces cerevisiae Proteins/biosynthesis
- Saccharomyces cerevisiae Proteins/genetics*
- Transcription Factors/biosynthesis
- Transcription Factors/genetics*
- Transgenes
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 17369834 Full text @ Nat. Methods
Citation
Scott, E.K., Mason, L., Arrenberg, A.B., Ziv, L., Gosse, N.J., Xiao, T., Chi, N.C., Asakawa, K., Kawakami, K., and Baier, H. (2007) Targeting neural circuitry in zebrafish using GAL4 enhancer trapping. Nature Methods. 4(4):323-326.
Abstract
We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping