PUBLICATION

Dynamic somite cell rearrangements lead to distinct waves of myotome growth

Authors
Stellabotte, F., Dobbs-McAuliffe, B., Fernandez, D.A., Feng, X., and Devoto, S.H.
ID
ZDB-PUB-070303-30
Date
2007
Source
Development (Cambridge, England)   134(7): 1253-1257 (Journal)
Registered Authors
Devoto, Stephen Henri, Dobbs-McAuliffe, Betsy, Stellabotte, Frank
Keywords
Myotome, Anterior border cells, Cell fate, Paraxial mesoderm, Somite, Dermomyotome, Zebrafish, Fast muscle, Pax7, Myogenesis, Teleost
MeSH Terms
  • Animals
  • Body Weights and Measures
  • Cell Lineage/physiology
  • Immunohistochemistry
  • Microscopy, Confocal
  • Muscle Development/physiology*
  • Myoblasts/physiology*
  • Somites/physiology*
  • Zebrafish/embryology*
PubMed
17314134 Full text @ Development
Abstract
The myogenic precursors responsible for muscle growth in amniotes develop from the dermomyotome, an epithelium at the external surface of the somite. In teleosts, the myogenic precursors responsible for growth have not been identified. We have used single cell lineage labeling in zebrafish to show that anterior border cells of epithelial somites are myogenic precursors responsible for zebrafish myotome growth. These cells move to the external surface of the embryonic myotome and express the transcription factor Pax7. Some remain on the external surface and some incorporate into the fast myotome, apparently by moving between differentiated slow fibres. The posterior cells of the somite, by contrast, elongate into medial muscle fibres. The surprising movement of the anterior somite cells to the external somite surface transforms a segmentally repeated arrangement of myogenic precursors into a medio-lateral arrangement similar to that seen in amniotes.
Genes / Markers
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping