PUBLICATION
Behavioral phenotyping in zebrafish: comparison of three behavioral quantification methods
- Authors
- Blaser, R., and Gerlai, R.
- ID
- ZDB-PUB-061229-28
- Date
- 2006
- Source
- Behavior research methods 38(3): 456-469 (Journal)
- Registered Authors
- Gerlai, Robert T.
- Keywords
- none
- MeSH Terms
-
- Aggression/physiology*
- Analysis of Variance
- Animals
- Behavior, Animal/physiology*
- Behavioral Sciences/methods
- Escape Reaction/physiology
- Exploratory Behavior/physiology*
- Female
- Male
- Motor Activity/physiology
- Phenotype*
- Reference Values
- Social Behavior*
- Statistics, Nonparametric
- Zebrafish
- PubMed
- 17186756 Full text @ Behav. Res. Methods
Citation
Blaser, R., and Gerlai, R. (2006) Behavioral phenotyping in zebrafish: comparison of three behavioral quantification methods. Behavior research methods. 38(3):456-469.
Abstract
The zebrafish has been popular in developmental biology and genetics, but its brain function has rarely been studied. High-throughput screening of mutation or drug-induced changes in brain function requires simple and automatable behavioral tests. This article compares three behavioral quantification methods in four simple behavioral paradigms that test a range of characteristics of adult zebrafish, including novelty-induced responses, social behavior, aggression, and predator-model-induced responses. Two quantification methods, manual recording and computerized videotracking of location and activity, yielded very similar results, suggesting that automated videotracking reliably measures activity parameters and will allow high-throughput screening. However, observation-based event recording of posture patterns was found generally not to correlate with videotracking measures, suggesting that further refinement of automated behavior quantification may be considered.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping