PUBLICATION
The zebrafish genome contains two inducible, functional cyclooxygenase-2 genes
- Authors
- Ishikawa, T.O., Griffin, K.J., Banerjee, U., and Herschman, H.R.
- ID
- ZDB-PUB-061205-4
- Date
- 2007
- Source
- Biochemical and Biophysical Research Communications 352(1): 181-187 (Journal)
- Registered Authors
- Griffin, Kevin
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- COS Cells
- Cell Line
- Chlorocebus aethiops
- Conserved Sequence
- Cyclooxygenase 2/chemistry
- Cyclooxygenase 2/genetics*
- Cyclooxygenase 2/metabolism*
- Genome/genetics*
- Isoenzymes/chemistry
- Isoenzymes/genetics
- Isoenzymes/metabolism
- Molecular Sequence Data
- Phylogeny
- Sequence Alignment
- Zebrafish/genetics*
- PubMed
- 17112475 Full text @ Biochem. Biophys. Res. Commun.
Citation
Ishikawa, T.O., Griffin, K.J., Banerjee, U., and Herschman, H.R. (2007) The zebrafish genome contains two inducible, functional cyclooxygenase-2 genes. Biochemical and Biophysical Research Communications. 352(1):181-187.
Abstract
Cyclooxygenase is a key enzyme in prostanoid biosynthesis. Mammalian species have two cyclooxygenases, constitutively expressed cyclooxygenase-1 (Cox-1) and inducible cyclooxygenase-2 (Cox-2). Cox-1 and/or Cox-2 have been also identified in other vertebrates, including fish. We identified a second zebrafish Cox-2 gene orthologue, Cox-2b. All of the functionally important amino acids for cyclooxygenase enzymes are conserved in Cox-2b. The 3' untranslated region of the Cox-2b message contains AU rich elements characteristic of regulation at the level of mRNA stability. Constitutive tissue expression patterns for Cox-2a and Cox-2b are distinct, but overlap. Both Cox-2a and Cox-2b expression are inducible in the kidney when fish are exposed to tetradecanoylphorbol acetate. Like Cox-2a, Cox-2b protein, expressed in COS cells is functionally active. Thus, the zebrafish genome contains two functional, inducible Cox-2 genes. Database searching demonstrates that some fish genomes contain multiple Cox-1 or Cox-2 cyclooxygenase genes, suggesting alternate duplication and retention of this gene.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping