PUBLICATION

Differential regulation of the zebrafish orthopedia1 gene during fate determination of diencephalic neurons

Authors
Del Giacco, L., Sordino, P., Pistocchi, A., Andreakis, N., Tarallo, R., Di Benedetto, B., and Cotelli, F.
ID
ZDB-PUB-061108-3
Date
2006
Source
BMC Developmental Biology   6: 50 (Journal)
Registered Authors
Cotelli, Franco, Del Giacco, Luca, Sordino, Paolo
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Diencephalon/growth & development*
  • Diencephalon/metabolism*
  • Gene Expression Regulation, Developmental/genetics
  • Gene Expression Regulation, Developmental/physiology*
  • Homeodomain Proteins/biosynthesis
  • Homeodomain Proteins/genetics
  • Molecular Sequence Data
  • Nerve Tissue Proteins/biosynthesis
  • Nerve Tissue Proteins/genetics
  • Neurons/cytology
  • Neurons/metabolism*
  • Neurons/physiology
  • Phylogeny
  • Protein Isoforms/biosynthesis
  • Protein Isoforms/genetics
  • Transcription Factors/biosynthesis*
  • Transcription Factors/genetics
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics
(all 24)
PubMed
17074092 Full text @ BMC Dev. Biol.
Abstract
ABSTRACT: BACKGROUND: The homeodomain transcription factor Orthopedia (Otp) is essential in restricting the fate of multiple classes of secreting neurons in the neuroendocrine hypothalamus of vertebrates. However, there is little information on the intercellular factors that regulate Otp expression during development. RESULTS: Here, we identified two otp orthologues in zebrafish (otp1 and otp2) and explored otp1 in the context of the morphogenetic pathways that specify neuroectodermal regions. During forebrain development, otp1 is expressed in anterior groups of diencephalic cells, positioned in the preoptic area (PO) (anterior alar plate) and the posterior tuberculum (PT) (posterior basal plate). The latter structure is characterized by Tyrosine Hydroxylase (TH)-positive cells, suggesting a role for otp1 in the lineage restriction of catecholaminergic (CA) neurons. Disruptions of Hedgehog (HH) and Fibroblast Growth Factor (FGF) pathways point to the ability of SHH protein to trigger otp1 expression in PO presumptive neuroblasts, with the attenuating effect of Dzip1 and FGF8. In addition, our data disclose otp1 as a determinant of CA neurons in the PT, where otp1 activity is strictly dependent on Nodal signaling and it is not responsive to SHH and FGF. CONCLUSIONS: In this study, we pinpoint the evolutionary importance of otp1 transcription factor in cell states of the diencephalon anlage and early neuronal progenitors. Furthermore, our data indicate that morphogenetic mechanisms differentially regulate otp1 expression in alar and basal plates.
Genes / Markers
Marker Marker Type Name
axin1GENEaxin 1
dzip1GENEDAZ interacting zinc finger protein 1
egr2bGENEearly growth response 2b
fgf8aGENEfibroblast growth factor 8a
med12GENEmediator complex subunit 12
neurog1GENEneurogenin 1
otpaGENEorthopedia homeobox a
otpbGENEorthopedia homeobox b
pax2aGENEpaired box 2a
ptch2GENEpatched 2
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Figures
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
egr2bABstandard conditions10-13 somitesISH
egr2bABstandard conditions10-13 somitesISH
fgf8aABstandard conditions5-9 somitesISH
fgf8aABstandard conditionsPrim-5ISH
fgf8aABstandard conditionsPrim-5ISH
fgf8aABstandard conditionsPrim-5ISH
neurog1ABstandard conditionsLong-pecISH
neurog1ABstandard conditionsLong-pecISH
neurog1AB + MO1-otpbstandard conditionsLong-pecISH
neurog1AB + MO1-otpbstandard conditionsLong-pecISH
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Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b16
    		Deficiency
    m807
      		Small Deletion
      tbx392
        		Point Mutation
        ti282a
          		Point Mutation
          tm213
            		Point Mutation
            ts294e
              		Point Mutation
              1 - 6 of 6
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              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              Target Reagent Reagent Type
              otpbMO1-otpbMRPHLNO
              1 - 1 of 1
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              Fish
              Fish
              AB
              AB + MO1-otpb
              axin1tm213/tm213
              Df(Chr12:hhex,ndr2)b16/b16
              dzip1ts294e/ts294e
              fgf8ati282a/ti282a
              fgf8ati282a/ti282a; shhatbx392/tbx392
              med12m807/m807
              shhatbx392/tbx392
              1 - 9 of 9
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              Antibodies
              No data available
              Orthology
              Gene Orthology
              otpa
              otpb
              1 - 2 of 2
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              Engineered Foreign Genes
              No data available
              Mapping
              No data available
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              Citation
              Del Giacco, L., Sordino, P., Pistocchi, A., Andreakis, N., Tarallo, R., Di Benedetto, B., and Cotelli, F. (2006) Differential regulation of the zebrafish orthopedia1 gene during fate determination of diencephalic neurons. BMC Developmental Biology. 6:50.