PUBLICATION

Tbx16 cooperates with Wnt11 in assembling the zebrafish organizer

Authors
Muyskens, J.B., and Kimmel, C.B.
ID
ZDB-PUB-061108-10
Date
2007
Source
Mechanisms of Development   124(1): 35-42 (Journal)
Registered Authors
Kimmel, Charles B., Muyskens, Jon
Keywords
Gastrulation, T-box transcription factors, Planar cell polarity, Live imaging, Protocadherin
MeSH Terms
  • Animals
  • Base Sequence
  • Body Patterning
  • Cadherins/genetics
  • Cadherins/metabolism
  • Gene Expression Regulation, Developmental
  • Oligodeoxyribonucleotides, Antisense/genetics
  • Oligodeoxyribonucleotides, Antisense/pharmacology
  • Organizers, Embryonic/embryology*
  • Organizers, Embryonic/metabolism
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Signal Transduction
  • T-Box Domain Proteins/deficiency
  • T-Box Domain Proteins/genetics
  • T-Box Domain Proteins/metabolism*
  • Wnt Proteins/deficiency
  • Wnt Proteins/genetics
  • Wnt Proteins/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 25)
PubMed
17081734 Full text @ Mech. Dev.
Abstract
The organizer, the signaling center that specifies vertebrate axial polarity and the nervous system, is a dorsal midline mesodermal domain in the gastrula that will form prechordal plate and anterior notochord. We show that in zebrafish the organizer is not a single domain when it first arises in the nascent mesoderm at the onset of gastrulation. Rather, in the presumptive prechordal plate region, the organizer is subdivided into two side-by-side cellular fields. Within minutes, concurrent medial and anterior cellular movements merge, or 'coalesce', the two fields to form the well-known singular midline field. Coalescence forms a symmetrical domain because the cell movements on the left and right sides initiate simultaneously and occur synchronously. However, in embryos with reduced function of the T-box transcription factor Tbx16 (Spadetail) or its genetic target paraxial protocadherin (Papc), synchrony is lost, coalesence is disrupted, and the midline domain is misshaped. Furthermore, with combined loss of Tbx16 and Wnt11 (Silberblick), coalesence is essentially absent. Possibly as a consequence, both the anterior movement of presumptive prechordal plate and organizer function, as assayed by eye-field separation, are disrupted. Our findings thus reveal that Tbx16, in combination with Wnt11, are critical components not only in morphogenesis but also in initial assembly of the organizer.
Genes / Markers
Marker Marker Type Name
dlx3bGENEdistal-less homeobox 3b
egr2bGENEearly growth response 2b
gscGENEgoosecoid
myod1GENEmyogenic differentiation 1
pax2aGENEpaired box 2a
pcdh8GENEprotocadherin 8
tbx16GENET-box transcription factor 16
tbxtaGENET-box transcription factor Ta
wnt11f2GENEwnt 11, family member 2
1 - 9 of 9
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Figures
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
dlx3btbx16b104/b104 (AB)standard conditionsBudISH
dlx3bwnt11f2tz216/tz216standard conditionsBudISH
dlx3bwnt11f2tz216/tz216; tbx16b104/b104standard conditionsBudISH
dlx3bWTstandard conditionsBudISH
egr2btbx16b104/b104 (AB)standard conditionsBudISH
egr2bwnt11f2tz216/tz216standard conditionsBudISH
egr2bwnt11f2tz216/tz216; tbx16b104/b104standard conditionsBudISH
egr2bWTstandard conditionsBudISH
gsctbx16b104/b104 (AB)standard conditionsBudISH
gscwnt11f2tz216/tz216standard conditionsBudISH
1 - 10 of 36
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Phenotype
Fish Conditions Stage Phenotype Figure
tbx16b104/b104 (AB)standard conditionsPrim-5
wnt11f2tz216/tz216standard conditionsPrim-5
wnt11f2tz216/tz216; tbx16b104/b104standard conditionsPrim-5
1 - 3 of 3
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b104
    		Indel
    tz216
      		Point Mutation
      1 - 2 of 2
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      Human Disease / Model
      Sequence Targeting Reagents
      Fish
      Antibodies
      Orthology
      Engineered Foreign Genes
      No data available
      Mapping
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      Citation
      Muyskens, J.B., and Kimmel, C.B. (2007) Tbx16 cooperates with Wnt11 in assembling the zebrafish organizer. Mechanisms of Development. 124(1):35-42.