PUBLICATION
A rapid, high content, in vivo model of glucocorticoid-induced osteoporosis
- Authors
- Barrett, R., Chappell, C., Quick, M., and Fleming, A.
- ID
- ZDB-PUB-060816-14
- Date
- 2006
- Source
- Biotechnology Journal 1(6): 651-655 (Journal)
- Registered Authors
- Fleming, Angeleen
- Keywords
- Glucocorticoid, Osteoporosis, Zebrafish, Animal model, High-throughput
- MeSH Terms
-
- Animals
- Calcification, Physiologic/drug effects*
- Diphosphonates/administration & dosage*
- Disease Models, Animal*
- Glucocorticoids*
- Osteoporosis/chemically induced*
- Osteoporosis/drug therapy*
- Osteoporosis/pathology
- Treatment Outcome
- Zebrafish*
- PubMed
- 16892313 Full text @ Biotechnol. J.
Citation
Barrett, R., Chappell, C., Quick, M., and Fleming, A. (2006) A rapid, high content, in vivo model of glucocorticoid-induced osteoporosis. Biotechnology Journal. 1(6):651-655.
Abstract
Glucocorticoid-induced osteoporosis (GIOP) is a major clinical problem given the widespread use of steroids and limited efficacy of biphosphonates. Existing animal models of GIOP are both slow and expensive. Hence, there is a need both for adjunctive modelling systems, as well as more efficacious therapies for the treatment of GIOP. We have addressed this issue through the creation of a zebrafish model of GIOP, which can be used for 96-well plate in vivo screening with an assay time of 5 days. The model demonstrates key similarities to human GIOP including a partial response to bisphosphonates. The ability to extract detailed pharmacological data, including concentration-response analyses, enables the screening and ranking of candidate therapeutic compounds. In addition, the zebrafish model is highly relevant for pathway dissection through genetic knockdown and overexpression studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping