PUBLICATION

Assignment of zebrafish genetic linkage groups to chromosomes

Authors
Phillips, R.B., Amores, A., Morasch, M.R., Wilson, C., and Postlethwait, J.H.
ID
ZDB-PUB-060719-6
Date
2006
Source
Cytogenetic and genome research   114(2): 155-162 (Journal)
Registered Authors
Amores, Angel, Phillips, Ruth B., Postlethwait, John H., Wilson, Catherine
Keywords
none
MeSH Terms
  • Animals
  • Cell Line
  • Chromosome Mapping
  • Chromosomes/genetics*
  • Genetic Linkage/genetics*
  • Humans
  • Karyotyping
  • Zebrafish/genetics*
PubMed
16825768 Full text @ Cytogenet. Genome Res.
Abstract
In this report the zebrafish genetic linkage groups are assigned to specific chromosomes using fluorescence in situ hybridization (FISH) with BAC probes containing genes mapped to each linkage group (LG). Chromosomes were identified using a combination of relative size and arm ratios. The largest genetic maps generally corresponded to the largest chromosomes, but genetic recombination tended to be elevated in the smaller chromosomes and near telomeres. Large insert clones containing genes near telomeres often hybridized to telomeres of multiple chromosome pairs, suggesting the presence of shared subtelomeric repetitive DNAs near telomeres. Evidence from comparative gene mapping in medaka, zebrafish, pufferfish, and humans suggests that the linkage groups of these species have the content of duplicate proto-chromosomes. However, these duplicate linkage groups are not associated with chromosomes of similar size or morphology. This suggests that considerable chromosome restructuring occurred subsequent to the genome duplication in teleosts.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping