PUBLICATION

Zebrafish msxB, msxC and msxE function together to refine the neural-nonneural border and regulate cranial placodes and neural crest development

Authors
Phillips, B.T., Kwon, H.J., Melton, C., Houghtaling, P., Fritz, A., and Riley, B.B.
ID
ZDB-PUB-060501-3
Date
2006
Source
Developmental Biology   294(2): 376-390 (Journal)
Registered Authors
Kwon, Hye-Joo, Riley, Bruce
Keywords
msx, dlx, eya, six, foxd3, snail2, sox10, Placode, Neural crest
MeSH Terms
  • Animals
  • Cell Proliferation
  • Cell Survival
  • Embryo, Nonmammalian/anatomy & histology
  • Embryo, Nonmammalian/physiology
  • Embryonic Structures/anatomy & histology
  • Embryonic Structures/physiology*
  • Gene Expression Regulation, Developmental
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism*
  • In Situ Hybridization
  • Neural Crest/cytology
  • Neural Crest/embryology*
  • Neurons/cytology
  • Neurons/physiology*
  • Oligonucleotides, Antisense/genetics
  • Oligonucleotides, Antisense/metabolism
  • Phenotype
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 25)
PubMed
16631154 Full text @ Dev. Biol.
Abstract
The zebrafish muscle segment homeobox genes msxB, msxC and msxE are expressed in partially overlapping domains in the neural crest and preplacodal ectoderm. We examined the roles of these msx genes in early development. Disrupting individual msx genes causes modest variable defects, whereas disrupting all three produces a reproducible severe phenotype, suggesting functional redundancy. Neural crest differentiation is blocked at an early stage. Preplacodal development begins normally, but placodes arising from the msx expression domain later show elevated apoptosis and are reduced in size. Cell proliferation is normal in these tissues. Unexpectedly, Msx-deficient embryos become ventralized by late gastrulation whereas misexpression of msxB dorsalizes the embryo. These effects appear to involve Distal-less (Dlx) protein activity, as loss of dlx3b and dlx4b suppresses ventralization in Msx-depleted embryos. At the same time, Msx-depletion restores normal preplacodal gene expression to dlx3b-dlx4b mutants. These data suggest that mutual antagonism between Msx and Dlx proteins achieves a balance of function required for normal preplacodal differentiation and placement of the neural-nonneural border.
Genes / Markers
Marker Marker Type Name
dlx3bGENEdistal-less homeobox 3b
dlx4bGENEdistal-less homeobox 4b
egr2bGENEearly growth response 2b
eya1GENEEYA transcriptional coactivator and phosphatase 1
fgf8aGENEfibroblast growth factor 8a
foxd3GENEforkhead box D3
foxi1GENEforkhead box i1
hoxb3aGENEhomeobox B3a
lef1GENElymphoid enhancer-binding factor 1
msx1aGENEmuscle segment homeobox 1a
1 - 10 of 22
Show
Figures
Figure Gallery (8 images)
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
AB + MO1-msx1astandard conditionsPrim-15
AB + MO1-msx1astandard conditionsPrim-15
AB + MO1-msx1astandard conditionsProtruding-mouth
AB + MO1-msx1astandard conditionsProtruding-mouth
AB + MO1-msx1astandard conditionsProtruding-mouth
AB + MO1-msx1a + MO1-msx3 + MO4-msx1bstandard conditions5-9 somites
AB + MO1-msx1a + MO1-msx3 + MO4-msx1bstandard conditions5-9 somites
AB + MO1-msx1a + MO1-msx3 + MO4-msx1bstandard conditions10-13 somites
AB + MO1-msx1a + MO1-msx3 + MO4-msx1bstandard conditions10-13 somites
AB + MO1-msx1a + MO1-msx3 + MO4-msx1bstandard conditions10-13 somites
1 - 10 of 39
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b380
    		Deficiency
    x8
      		Deficiency
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      msx1aMO1-msx1aMRPHLNO
      msx1bMO4-msx1bMRPHLNO
      msx3MO1-msx3MRPHLNO
      1 - 3 of 3
      Show
      Fish
      Fish
      AB
      AB + MO1-msx1a
      AB + MO1-msx1a + MO1-msx3 + MO4-msx1b
      AB + MO1-msx3
      AB + MO4-msx1b
      Df(Chr01:lef1,msxb)x8/x8
      Df(Chr01:lef1,msxb)x8/x8 + MO1-msx1a + MO1-msx3 + MO4-msx1b
      Df(Chr12:dlx3b,dlx4b,tbx6)b380/b380
      Df(Chr12:dlx3b,dlx4b,tbx6)b380/b380 + MO1-msx1a + MO1-msx3 + MO4-msx1b
      WT
      1 - 10 of 10
      Show
      Antibodies
      No data available
      Orthology
      Engineered Foreign Genes
      No data available
      Mapping
      Your Input Welcome
      We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
      Citation
      Phillips, B.T., Kwon, H.J., Melton, C., Houghtaling, P., Fritz, A., and Riley, B.B. (2006) Zebrafish msxB, msxC and msxE function together to refine the neural-nonneural border and regulate cranial placodes and neural crest development. Developmental Biology. 294(2):376-390.