PUBLICATION

Differential Roles of Transcriptional Mediator Complex Subunits Crsp34/Med27, Crsp150/Med14 and Trap100/Med24 During Zebrafish Retinal Development

Authors
Durr, K., Holzschuh, J., Filippi, A., Ettl, A.K., Ryu, S., Shepherd, I.T., and Driever, W.
ID
ZDB-PUB-060412-6
Date
2006
Source
Genetics   174(2): 693-705 (Journal)
Registered Authors
Driever, Wolfgang, Duerr, Katrin, Ettl, Anne-Kathrin, Filippi, Alida, Holzschuh, Jochen, Ryu, Soojin, Shepherd, Iain T.
Keywords
cell differentiation, mutational analysis, retina, transcriptional mediator complex, zebrafish
MeSH Terms
  • Alleles
  • Amacrine Cells/cytology
  • Animals
  • Cell Differentiation/genetics
  • Gene Expression Regulation, Developmental
  • Mediator Complex
  • Phenotype
  • Protein Subunits/biosynthesis
  • Protein Subunits/genetics
  • Protein Subunits/physiology*
  • Retina/embryology*
  • Retina/metabolism
  • Trans-Activators/biosynthesis
  • Trans-Activators/genetics
  • Trans-Activators/physiology*
  • Zebrafish
  • Zebrafish Proteins/biosynthesis
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
(all 19)
PubMed
16582438 Full text @ Genetics
Abstract
The transcriptional mediator complex has emerged as an important component of transcriptional regulation, yet it is largely unknown whether its subunits have differential functions in development. We demonstrate that the zebrafish mutation m885 disrupts a subunit of the mediator complex, Crsp34/Med27. In order to explore the role of the mediator in the control of retinal differentiation, we employed two additional mutations disrupting the mediator subunits Trap100/Med24 and Crsp150/Med14. Our analysis shows that loss of Crsp34/Med27 decreases amacrine cell number, but increases the number of rod photoreceptor cells. In contrast, loss of Trap100/Med24 decreases rod photoreceptor cells. Loss of Crsp150/Med14, on the other hand, only slightly reduces dopaminergic amacrine cells, which are absent from both crsp34(m885) and trap100(lessen) mutant embryos. Our data provide evidence for differential requirements for Crsp34/Med27 in developmental processes. In addition, our data point to divergent functions of the mediator subunits Crsp34/Med27, Trap100/Med24 and Crsp150/Med14, and thus, suggest that subunit composition of the mediator contributes to the control of differentiation in the vertebrate CNS.
Genes / Markers
Marker Marker Type Name
mcm5GENEminichromosome maintenance complex component 5
med14GENEmediator complex subunit 14
med24GENEmediator complex subunit 24
med27GENEmediator complex subunit 27
opn1lw1GENEopsin 1 (cone pigments), long-wave-sensitive, 1
pax6bGENEpaired box 6b
rhoGENErhodopsin
thGENEtyrosine hydroxylase
tph1aGENEtryptophan hydroxylase 1 (tryptophan 5-monooxygenase) a
1 - 9 of 9
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Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hi2143TgTransgenic Insertion
m885
    		Point Mutation
    w24
      		Point Mutation
      1 - 3 of 3
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      med27MO1-med27MRPHLNO
      1 - 1 of 1
      Show
      Fish
      Fish
      AB/TL
      AB/TL + MO1-med27
      med14hi2143Tg
      med24w24/w24
      med27m885/m885
      1 - 5 of 5
      Show
      Antibodies
      Orthology
      Engineered Foreign Genes
      No data available
      Mapping
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      Citation
      Durr, K., Holzschuh, J., Filippi, A., Ettl, A.K., Ryu, S., Shepherd, I.T., and Driever, W. (2006) Differential Roles of Transcriptional Mediator Complex Subunits Crsp34/Med27, Crsp150/Med14 and Trap100/Med24 During Zebrafish Retinal Development. Genetics. 174(2):693-705.