PUBLICATION
Two GC-rich boxes in huC promoter play distinct roles in controlling its neuronal specific expression in zebrafish embryos
- Authors
- Zhao, C., He, X., Tian, C., and Meng, A.
- ID
- ZDB-PUB-060216-5
- Date
- 2006
- Source
- Biochemical and Biophysical Research Communications 342(1): 214-220 (Journal)
- Registered Authors
- Meng, Anming, Zhao, Chengtian
- Keywords
- Zebrafish, huC, Neuron, GC-rich box, Sp1-like transcription factors
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- ELAV Proteins/genetics*
- ELAV Proteins/metabolism*
- ELAV-Like Protein 3
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- GC Rich Sequence/genetics*
- Gene Expression Regulation, Developmental
- Genes, Reporter/genetics
- Molecular Sequence Data
- Neurons/metabolism*
- Organ Specificity
- Promoter Regions, Genetic/genetics*
- Sp1 Transcription Factor/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism*
- PubMed
- 16472769 Full text @ Biochem. Biophys. Res. Commun.
Citation
Zhao, C., He, X., Tian, C., and Meng, A. (2006) Two GC-rich boxes in huC promoter play distinct roles in controlling its neuronal specific expression in zebrafish embryos. Biochemical and Biophysical Research Communications. 342(1):214-220.
Abstract
HuC, a vertebrate ortholog of Drosophila elav gene, encodes an RNA binding protein and is involved in early neurogenesis. Zebrafish huC is expressed in distinct neurons, including Rohon-Beard (RB) sensory neurons, interneurons and motoneurons, during primary neurogenesis, and in all neurons later during secondary neurogenesis. In this study, we identify two GC-rich box elements, proximal GC (p-GC) box from -172 to -149 and distal GC (d-GC) box from -218 to -208, in zebrafish huC promoter. Using transgenic approach, we demonstrate that deletion of the p-GC box from the promoter results in loss of expression of the reporter GFP in neurons while deletion of the d-GC box leads to GFP expression only in dorsal RB sensory neurons. These results suggest that the p-GC box alone confers transcriptional activity of huC promoter in primary RB neurons and the d-GC is required for huC transcription in the full spectrum of spinal cord neurons. Further studies are needed to identify specific Sp1-like transcription factors that bind to these GC boxes and activate huC transcription.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping