PUBLICATION
In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes
- Authors
- Kloosterman, W.P., Wienholds, E., de Bruijn, E., Kauppinen, S., and Plasterk, R.H.
- ID
- ZDB-PUB-060105-3
- Date
- 2006
- Source
- Nature Methods 3(1): 27-29 (Journal)
- Registered Authors
- de Bruijn, Ewart, Kloosterman, Wigard, Plasterk, Ronald H.A., Wienholds, Erno
- Keywords
- none
- MeSH Terms
-
- Animals
- Embryo, Mammalian/chemistry*
- Embryo, Nonmammalian/chemistry
- Gene Expression Profiling/methods
- In Situ Hybridization/methods*
- Mice
- MicroRNAs/analysis*
- Oligonucleotide Probes/chemistry*
- Oligonucleotides
- Oligonucleotides, Antisense/chemistry*
- Zebrafish/genetics
- PubMed
- 16369549 Full text @ Nat. Methods
Citation
Kloosterman, W.P., Wienholds, E., de Bruijn, E., Kauppinen, S., and Plasterk, R.H. (2006) In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes. Nature Methods. 3(1):27-29.
Abstract
MicroRNAs (miRNAs) are 20-23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping