PUBLICATION
Characterization of NPY receptor subtypes Y2 and Y7 in rainbow trout Oncorhynchus mykiss
- Authors
- Larsson, T.A., Larson, E.T., Fredriksson, R., Conlon, J.M., and Larhammar, D.
- ID
- ZDB-PUB-051221-9
- Date
- 2006
- Source
- Peptides 27(6): 1320-1327 (Journal)
- Registered Authors
- Fredriksson, Robert, Larhammar, Dan, Larson, Earl T., Larsson, Tomas A.
- Keywords
- NPY, Y2, Y7, Rainbow trout, G-protein coupled receptor
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Chickens
- Dose-Response Relationship, Drug
- Gene Library
- Humans
- Kinetics
- Molecular Sequence Data
- Neuropeptide Y/chemistry
- Oncorhynchus mykiss/metabolism*
- Phylogeny
- Polymerase Chain Reaction
- Protein Binding
- Receptors, Neuropeptide Y/chemistry*
- Receptors, Neuropeptide Y/metabolism
- Sequence Homology, Amino Acid
- PubMed
- 16359756 Full text @ Peptides
Citation
Larsson, T.A., Larson, E.T., Fredriksson, R., Conlon, J.M., and Larhammar, D. (2006) Characterization of NPY receptor subtypes Y2 and Y7 in rainbow trout Oncorhynchus mykiss. Peptides. 27(6):1320-1327.
Abstract
We report the cloning and pharmacological characterization of two neuropeptide Y (NPY) receptor subtypes, Y2 and Y7, in rainbow trout (Oncorhynchus mykiss). These subtypes are approximately 50% identical to each other and belong to the Y2 subfamily of NPY receptors. The binding properties of the receptors were investigated after expression in human HEK-293 EBNA cells. Both receptors bound the three zebrafish peptides NPY, PYYa, and PYYb, as well as porcine NPY and PYY, with affinities in the nanomolar range that are similar to mammalian Y2. The affinity of the truncated porcine NPY fragments, NPY 13-36 and NPY 18-36 was markedly lower compared to mammalian and chicken Y2. This suggests that mammalian and chicken Y2 are unique among NPY receptors in their ability to bind truncated peptide fragments. The antagonist BIIE0246, developed for mammalian Y2, did not bind either of the two rainbow trout receptors. Our results support the proposed expansion of this gene family by duplications before the gnathostome radiation. They also reveal appreciable differences in the repertoire and characteristics of NPY receptors between fish and tetrapods stressing the importance of lineage-specific gene loss as well as sequence divergence after duplication.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping