PUBLICATION
Mutation in sodium-calcium exchanger 1 (NCX1) causes cardiac fibrillation in zebrafish
- Authors
- Langenbacher, A.D., Dong, Y., Shu, X., Choi, J., Nicoll, D.A., Goldhaber, J.I., Philipson, K.D., and Chen, J.N.
- ID
- ZDB-PUB-051207-9
- Date
- 2005
- Source
- Proceedings of the National Academy of Sciences of the United States of America 102(49): 17699-17704 (Journal)
- Registered Authors
- Chen, Jau-Nian, Choi, Jayoung, Langenbacher, Adam, Shu, Xiaodong
- Keywords
- calcium homeostasis, cardiac arrhythmia, heart
- MeSH Terms
-
- Animals
- Arrhythmias, Cardiac/genetics*
- Arrhythmias, Cardiac/physiopathology*
- Base Sequence
- Calcium/metabolism
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Homeostasis
- Molecular Sequence Data
- Mutation/genetics*
- Myocardial Contraction/genetics
- Myocardial Contraction/physiology
- Phenotype
- Phylogeny
- RNA/genetics
- Sodium-Calcium Exchanger/genetics*
- Sodium-Calcium Exchanger/metabolism*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 16314583 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Langenbacher, A.D., Dong, Y., Shu, X., Choi, J., Nicoll, D.A., Goldhaber, J.I., Philipson, K.D., and Chen, J.N. (2005) Mutation in sodium-calcium exchanger 1 (NCX1) causes cardiac fibrillation in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 102(49):17699-17704.
Abstract
Cardiac fibrillation, a form of cardiac arrhythmia, is the most common cause of embolic stroke and death associated with heart failure. The molecular mechanisms underlying cardiac fibrillation are largely unknown. Here we report a zebrafish model for cardiac fibrillation. The hearts of zebrafish tremblor (tre) mutants exhibit chaotic movements and fail to develop synchronized contractions. Calcium imaging showed that normal calcium transients are absent in tre cardiomyocytes, and molecular cloning of the tre mutation revealed that the tre locus encodes the zebrafish cardiac-specific sodium-calcium exchanger (NCX) 1, NCX1h. Forced expression of NCX1h or other calcium-handling molecules restored synchronized heartbeats in tre mutant embryos in a dosage-dependent manner, demonstrating the critical role of calcium homeostasis in maintaining embryonic cardiac function. By creating mosaic zebrafish embryos, we showed that sporadic NCX1h-null cells were not sufficient to disrupt normal cardiac function, but clustered wild-type cardiomyocytes contract in unison in tre mutant hearts. These data signify the essential role of calcium homeostasis and NCX1h in establishing rhythmic contraction in the embryonic zebrafish heart.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping