PUBLICATION

Retinoic acid activates myogenesis in vivo through Fgf8 signalling

Authors
Hamade, A., Deries, M., Begemann, G., Bally-Cuif, L., Genet, C., Sabatier, F., Bonnieu, A., and Cousin, X.
ID
ZDB-PUB-051207-10
Date
2006
Source
Developmental Biology   289(1): 127-140 (Journal)
Registered Authors
Bally-Cuif, Laure, Begemann, Gerrit, Bonnieu, Anne, Cousin, Xavier, Genet, Carine, Hamade, Aline
Keywords
Retinoic acid, Myogenesis, Somites, Presomitic mesoderm, myoD, fgf8, acerebellar, Zebrafish
MeSH Terms
  • Animals
  • Embryo, Nonmammalian/drug effects
  • Fibroblast Growth Factor 8/genetics
  • Fibroblast Growth Factor 8/physiology*
  • Gene Expression Regulation, Developmental
  • Muscle Development*/genetics
  • MyoD Protein/analysis
  • MyoD Protein/metabolism
  • Protein Biosynthesis
  • Signal Transduction
  • Somites/chemistry
  • Somites/metabolism
  • Tretinoin/pharmacology
  • Tretinoin/physiology*
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
(all 17)
PubMed
16316642 Full text @ Dev. Biol.
CTD
16316642
Abstract
Retinoic acid (RA) has been shown to regulate muscle differentiation in vitro. Here, we have investigated the role of RA signalling during embryonic myogenesis in zebrafish. We have altered RA signalling from gastrulation stages onwards by either inhibiting endogenous RA synthesis using an inhibitor of retinaldehyde dehydrogenases (DEAB) or by addition of exogenous RA. DEAB reduces expression of the myogenic markers myoD and myogenin in somites, whereas RA induces increased expression of these genes and strongly induces premature myoD expression in the presomitic mesoderm (psm). The expression dynamics of myf5 in presomitic and somitic mesoderm suggest that RA promotes muscle differentiation, a role supported by the fact that RA activates expression of fast myosin, while DEAB represses it. We identify Fgf8 as a major relay factor in RA-mediated activation of myogenesis. We show that fgf8 expression in somites and anterior psm is regulated by RA, and find that in the absence of Fgf8 signalling in the acerebellar mutant RA fails to promote myoD expression. We propose that, in the developing embryo, localised synthesis of RA by Raldh2 in the anterior psm and in somites activates fgf8 expression which in turn induces the expression of myogenic genes and fast muscle differentiation.
Genes / Markers
Marker Marker Type Name
aldh1a2GENEaldehyde dehydrogenase 1 family, member A2
cyp26a1GENEcytochrome P450, family 26, subfamily A, polypeptide 1
egr2bGENEearly growth response 2b
etv5bGENEETS variant transcription factor 5b
fgf17GENEfibroblast growth factor 17
fgf8aGENEfibroblast growth factor 8a
mespbaGENEmesoderm posterior ba
myf5GENEmyogenic factor 5
myod1GENEmyogenic differentiation 1
myogGENEmyogenin
1 - 10 of 15
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Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b641
    		Point Mutation
    i26
      		Point Mutation
      ti282a
        		Point Mutation
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        aldh1a2i26/i26
        fgf8ati282a/ti282a
        smob641/b641 (AB)
        WT
        1 - 4 of 4
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        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        No data available
        Mapping
        No data available
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        Citation
        Hamade, A., Deries, M., Begemann, G., Bally-Cuif, L., Genet, C., Sabatier, F., Bonnieu, A., and Cousin, X. (2006) Retinoic acid activates myogenesis in vivo through Fgf8 signalling. Developmental Biology. 289(1):127-140.