PUBLICATION
Structure and expression of the zebrafish mest gene, an ortholog of mammalian imprinted gene PEG1/MEST
- Authors
- Hahn, Y., Yang, S.K., and Chung, J.H.
- ID
- ZDB-PUB-051107-34
- Date
- 2005
- Source
- BBA Gene Structure and Expression 1731(2): 125-132 (Journal)
- Registered Authors
- Keywords
- Mest, Zebrafish, Developmental expression, Allelic expression, One-codon alternative splicing
- MeSH Terms
-
- Alternative Splicing
- Amino Acid Sequence
- Animals
- Base Sequence
- Codon/metabolism
- DNA, Complementary/genetics
- Genetic Linkage
- Genomic Imprinting
- Molecular Sequence Data
- Proteins/genetics*
- Sequence Homology
- Synteny
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 16263186 Full text @ BBA Gene Structure and Expression
Citation
Hahn, Y., Yang, S.K., and Chung, J.H. (2005) Structure and expression of the zebrafish mest gene, an ortholog of mammalian imprinted gene PEG1/MEST. BBA Gene Structure and Expression. 1731(2):125-132.
Abstract
PEG1/MEST is a paternally expressed gene in placental mammals. Here, we report identification of zebrafish (Danio rerio) gene mest, an ortholog of mammalian PEG1/MEST. Zebrafish mest encodes a polypeptide of 344 amino acids and shows a significant similarity to mammalian orthologs. Zebrafish mest is present as a single copy in the zebrafish genome and is closely linked to copg2 as in mammals. It is notable that 10 of 11 intron positions in mest are conserved among mammalian PEG1/MEST genes, indicating that the genomic organization and linkage between mest and copg2 loci was established in ancient vertebrates. Zebrafish mest is expressed in blastula, segmentation, and larval stages, exhibiting gradually increased expression as the development proceeds. Allelic expression analysis in hybrid larvae shows that both parental alleles are transcribed. We also observed one-codon alternative splicing involving an alternative usage of the two consecutive splice acceptors of intron 1, generating two protein isoforms with different lengths of a single amino acid.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping