PUBLICATION
Zebrafish protocadherin 10 is involved in paraxial mesoderm development and somitogenesis
- Authors
- Murakami, T., Hijikata, T., Matsukawa, M., Ishikawa, H., and Yorifuji, H.
- ID
- ZDB-PUB-051107-23
- Date
- 2006
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 235(2): 506-514 (Journal)
- Registered Authors
- Murakami, Tohru
- Keywords
- protocadherin 10, paraxial protocadherin, paraxial mesoderm, somitogenesis, adaxial cells, Danio rerio, molecular cloning, in situ hybridization, morpholino
- MeSH Terms
-
- Animals
- Cadherins/genetics
- Cadherins/metabolism*
- Cell Adhesion
- Central Nervous System/embryology
- Central Nervous System/metabolism
- Cloning, Molecular
- DNA, Complementary/genetics
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental
- Head/embryology
- Mesoderm/cytology
- Mesoderm/metabolism*
- Somites/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins
- PubMed
- 16261626 Full text @ Dev. Dyn.
Citation
Murakami, T., Hijikata, T., Matsukawa, M., Ishikawa, H., and Yorifuji, H. (2006) Zebrafish protocadherin 10 is involved in paraxial mesoderm development and somitogenesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(2):506-514.
Abstract
Here, we present the first report of the molecular cloning of zebrafish protocadherin 10 (Pcdh10, OL-protocadherin) and describe its functional analyses in the development of segmental plate. Epitope-tagged Pcdh10 expressed in embryos was localized on cell peripheries of adjacent cells. In situ hybridization showed that pcdh10 was expressed in the paraxial mesoderm (PAM) and developing somites, and in the pineal body, the diencephalon, and the vicinity of otocysts. Expression in PAM increased in the last few presumptive somites, reached the maximum level in the latest segmenting somites, and decreased thereafter during somite maturation. These expression patterns suggested that Pcdh10 is involved in development of PAM and somites. This was confirmed by morpholino knockdown and dominant-negative inhibition of Pcdh10 in embryos, which disturbed movements of PAM cells and somite segmentation. Comparative studies showed that pcdh10 expression lasted up to approximately three times longer in maturing somites than that of paraxial protocadherin (pcdh8). They also indicated that the adaxial cells expressed pcdh8 but not pcdh10. We propose that Pcdh10 is involved in the morphogenic movements of PAM cells and somite segmentation and that differential adhesion of Pcdh8 and Pcdh10 plays a role in the morphogenic machinery of somites and adaxial cells. Developmental Dynamics, 2005. (c) 2005 Wiley-Liss, Inc.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping