PUBLICATION
Characterization and expression analysis of TNF-related apoptosis inducing ligand (TRAIL) in grass carp Ctenopharyngodon idella
- Authors
- Chang, M.X., Nie, P., Xie, H.X., Wang, G.L., and Gao, Y.
- ID
- ZDB-PUB-051012-24
- Date
- 2006
- Source
- Veterinary Immunology and Immunopathology 110(1-2): 51-63 (Journal)
- Registered Authors
- Nie, Pin
- Keywords
- TRAIL, Genome, Promoter, Expression, Grass carp
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Apoptosis Regulatory Proteins/biosynthesis*
- Apoptosis Regulatory Proteins/genetics
- Apoptosis Regulatory Proteins/immunology
- Base Sequence
- Carps/genetics
- Carps/immunology
- Carps/metabolism*
- Cloning, Molecular
- DNA, Complementary/genetics
- Membrane Glycoproteins/biosynthesis*
- Membrane Glycoproteins/genetics
- Membrane Glycoproteins/immunology
- Molecular Sequence Data
- Phylogeny
- Promoter Regions, Genetic
- Reverse Transcriptase Polymerase Chain Reaction/veterinary
- Sequence Alignment
- Sequence Analysis, DNA
- TNF-Related Apoptosis-Inducing Ligand
- Tumor Necrosis Factor-alpha/biosynthesis*
- Tumor Necrosis Factor-alpha/genetics
- Tumor Necrosis Factor-alpha/immunology
- PubMed
- 16216337 Full text @ Vet. Immunol. Immunopathol.
Citation
Chang, M.X., Nie, P., Xie, H.X., Wang, G.L., and Gao, Y. (2006) Characterization and expression analysis of TNF-related apoptosis inducing ligand (TRAIL) in grass carp Ctenopharyngodon idella. Veterinary Immunology and Immunopathology. 110(1-2):51-63.
Abstract
TRAIL (Apo2 ligand) described as a type II transmembrane protein belonging to the TNF superfamily can induce apoptotic cell death in a variety of cell types. In the present study, a putative cDNA sequence encoding the 299 amino acids of TRAIL (GC-TRAIL) and its genomic organization were identified in grass carp Ctenopharyngodon idella. The predicted GC-TRAIL sequence showed 44 and 41% identities to chicken and human TRAILs, respectively. In a domain search, a tumor necrosis factor homology domain (THD) was identified in the C-terminal portion of TRAILs. The GC-TRAIL gene consists of five exons, with four intervening introns, spaced over approximately 4kb of genomic sequence. Analysis of GC-TRAIL promoter region revealed the presence of a number of putative transcription factor binding sites, such as Sp1, NF-kappaB, AP-1, GATA, NFAT, HNF, STAT, P53 and IRF1 sequences which are important for the expression of other TNF family members. Phylogenetic analysis placed GC-TRAIL and the putative zebrafish (Danio rerio) TRAIL obtained from searching the zebrafish database into one separate cluster near mammalian TRAIL genes, but apart from the reported zebrafish TRAIL-like protein, indicating that the GC-TRAIL is an authentic fish TRAIL. Expression analysis revealed that GC-TRAIL is expressed in many tissues, such as in gills, liver, trunk kidney, head kidney, intestine and spleen.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping