PUBLICATION
Structural Analysis of cDNAs Coding for 4SNc-Tudor Domain Protein from Fish and Their Expression in Yellowtail Organs
- Authors
- Abe, S., Wang, P.L., Takahashi, F., and Sasaki, E.
- ID
- ZDB-PUB-050907-8
- Date
- 2005
- Source
- Marine biotechnology (New York, N.Y.) 7(6): 677-686 (Journal)
- Registered Authors
- Keywords
- SN4TDR, SND1, LRRC4, proteomics, torafugu, zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Brain/metabolism
- Chromatography, Affinity
- Chromatography, Liquid
- Cloning, Molecular
- DNA Primers
- DNA, Complementary/genetics
- Electrophoresis, Polyacrylamide Gel
- Fishes/genetics*
- Fishes/metabolism
- Gene Components
- Gills/metabolism
- Mass Spectrometry
- Molecular Sequence Data
- Protein Structure, Tertiary*
- Proteins/genetics*
- Proteins/metabolism*
- Sequence Analysis, DNA
- Viscera/metabolism*
- PubMed
- 16132464 Full text @ Mar. Biotechnol.
Citation
Abe, S., Wang, P.L., Takahashi, F., and Sasaki, E. (2005) Structural Analysis of cDNAs Coding for 4SNc-Tudor Domain Protein from Fish and Their Expression in Yellowtail Organs. Marine biotechnology (New York, N.Y.). 7(6):677-686.
Abstract
We cloned complementary DNAs for 4SNc-Tudor protein (SN4TDR) from yellowtail (Seriola quinqueradiata), torafugu (Takifugu rubripes), and zebrafish (Danio rerio). This protein contains 4 staphylococcal nuclease domains at the N terminus followed by a Tudor domain. We also identified the 4SNc-Tudor proteins highly homologous to that in yellowtail from the Takifugu genomic database. According to the smart database, these fish proteins had an overlapping Tudor domain (smart00333) with a complete 5 SNc domain (smart00318). In addition, 2 possible translation start sites were observed at the 5' sequences in all 3 fish species. Northern blot analysis of different yellowtail organs showed that the full SN4TDR messenger RNA was approximately 4000 nucleotides long and that its expression was highest in liver and gallbladder, being about 2 to 5 times higher than in kidney, brain, ovary, and gills, and exceedingly low in spleen, heart, and muscle. A minor 2000-nucleotide transcript observed in kidney, spleen, and gallbladder, was attributable to an alternatively spliced variant of this gene. Total proteins extracted from yellowtail liver were fractionated by heparin affinity column chromatography and separated by sodium dodecylsulfate polyacrylamide gel electrophoresis. Analyses by SDS-PAGE and liquid chromatography with tandem mass spectroscopy identified the polypeptide encoded by SN4TDR as a single molecule of 102 kDa.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping