PUBLICATION
Otolith matrix proteins OMP-1 and Otolin-1 are necessary for normal otolith growth and their correct anchoring onto the sensory maculae
- Authors
- Murayama, E., Herbomel, P., Kawakami, A., Takeda, H., and Nagasawa H.
- ID
- ZDB-PUB-050523-8
- Date
- 2005
- Source
- Mechanisms of Development 122(6): 791-803 (Journal)
- Registered Authors
- Herbomel, Philippe, Kawakami, Atsushi, Murayama, Emi, Takeda, Hiroyuki
- Keywords
- Biomineralization; Matrix protein; Morpholino; Otolith; Zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Calcium Carbonate/metabolism
- Cloning, Molecular
- DNA, Complementary/metabolism
- Ear, Inner/embryology
- Extracellular Matrix Proteins/physiology*
- Gene Expression Regulation, Developmental*
- In Situ Hybridization
- Microscopy, Video
- Molecular Sequence Data
- Nerve Tissue Proteins/physiology*
- Olfactory Marker Protein
- Oligonucleotides, Antisense/pharmacology
- Otolithic Membrane/embryology*
- Otolithic Membrane/physiology
- Phalloidine/pharmacology
- Phenotype
- Protein Structure, Tertiary
- RNA, Messenger/metabolism
- Time Factors
- Zebrafish
- Zebrafish Proteins
- PubMed
- 15905077 Full text @ Mech. Dev.
Citation
Murayama, E., Herbomel, P., Kawakami, A., Takeda, H., and Nagasawa H. (2005) Otolith matrix proteins OMP-1 and Otolin-1 are necessary for normal otolith growth and their correct anchoring onto the sensory maculae. Mechanisms of Development. 122(6):791-803.
Abstract
Fish otoliths are highly calcified concretions deposited in the inner ear and serve as a part of the hearing and balance systems. They consist mainly of calcium carbonate and a small amount of organic matrix. The latter component is considered to play important roles in otolith formation. Previously, we identified two major otolith matrix proteins, OMP-1 (otolith matrix protein-1) and Otolin-1, from salmonid species. To assess the function of these proteins in otolith formation, we performed antisense morpholino oligonucleotide (MO)-mediated knockdown of omp-1 and otolin-1 in zebrafish embryos. We first identified zebrafish cDNA homologs of omp-1 (zomp-1) and otolin-1 (zotolin-1). Whole-mount in situ hybridization then revealed that the expression of both zomp-1 and zotolin-1 mRNAs is restricted to the otic vesicles. zomp-1 mRNA was expressed from the 14-somite stage in the otic placode, but the zOMP-1 protein was detected only from 26-somite stage onwards. In contrast, zotolin-1 mRNA expression became clear around 72hpf. MOs designed to inhibit zomp-1 and zotolin-1 mRNA translation, respectively, were injected into 1-2 cell stage embryos. zomp-1 MO caused a reduction in otolith size and an absence of zOtolin-1 deposition, while zotolin-1 MO caused a fusion of the two otoliths, and an increased instability of otoliths after fixation. We conclude that zOMP-1 is required for normal otolith growth and deposition of zOtolin-1 in the otolith, while zOtolin-1, a collagenous protein, is involved in the correct arrangement of the otoliths onto the sensory epithelium of the inner ear and probably in stabilization of the otolith matrix.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping